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An experimental model of mycobacterial infection under corneal flaps BJMBR
Adan,C.B.D.; Sato,E.H.; Sousa,L.B.; Oliveira,R.S.; Leão,S.C.; Freitas,D..
In order to develop a new experimental animal model of infection with Mycobacterium chelonae in keratomileusis, we conducted a double-blind prospective study on 24 adult male New Zealand rabbits. One eye of each rabbit was submitted to automatic lamellar keratotomy with the automatic corneal shaper under general anesthesia. Eyes were immunosuppressed by a single local injection of methyl prednisolone. Twelve animals were inoculated into the keratomileusis interface with 1 µl of 10(6) heat-inactivated bacteria (heat-inactivated inoculum controls) and 12 with 1 µl of 10(6) live bacteria. Trimethoprim drops (0.1%, w/v) were used as prophylaxis for the surgical procedure every 4 h (50 µl, qid). Animals were examined by 2 observers under a slit lamp on the 1st,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Infectious eye diseases; Keratitis; Animal model; Mycobacterium chelonae; Lamellar keratotomy.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700010
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