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Immunohistochemical Study of Amelogenin and Lysosome-Associate Membrane Proteins (LAMPs) in Cartilage International Journal of Morphology
Hatakeyama,Yuji; Hatakeyama,Junko; Oka,Kyoko; Tsuruga,Eichi; Inai,Tetsuichiro; Anan,Hisashi; Sawa,Yoshihiko.
Amelogenin is one of the enamel matrix proteins secreted by ameloblasts during enamel formation in tooth development. Recent studies showed that the amelogenin is expressed in chondrocyte. Lysosome-associated membrane proteins (LAMPs) have been identified as binding partner proteins to amelogenin and it has been suggested they act as signaling receptors of amelogenin. The purpose of this study is to clarify the localization of amelogenin and LAMPs in growth plate cartilage and cartilaginous nodules in micromass culture. Mouse knee joints including tibia growth plate at 4 weeks old and micromass cultures of limb bud mesenchymal cells after 2 weeks were fixed in paraformaldehyde, routinely processed, sections were cut and immunostained with amelogenin,...
Tipo: Journal article Palavras-chave: Amelogenin; Lysosome-associated membrane proteins (LAMPs); Chondrogenesis; Cartilage.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200040
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