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| Scarcelli,Eliana; Piatti,Rosa Maria; Harakava,Ricardo; Miyashiro,Simone; Fernandes,Flora Maria de Campos; Campos,Fabíola Ribeiro; Francisco,Waldemar; Genovez,Margareth Élide; Richtzenhain,Leonardo José. |
| The objective of the present trial was to characterize genetically strains of Campylobacter jejuni subsp. jejuni isolated from humans and several animal sources (bovines, swine, dogs, primates, wild boars and poultry). A total of 828 different animal samples (feces, carcass, aborted fetus and hysterectomized uterus) were analysed by means of routine bacteriological methods, and 36 C. jejuni strains were isolated. Thirty strains of human fecal origin were obtained in clinical analysis laboratories in the city of São Paulo. The 66 C. jejuni strains isolated were submitted to genetic characterization. Primers based on fla A gene were used in a polymerase chain reaction (PCR) and amplified a fragment of the 702 bp. PCR products were evaluated by means of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni subtyping sequencing; Flagellin. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400014 |
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| Miyashiro,Simone; Scarcelli,Eliana; Piatti,Rosa M.; Campos,Fabíola R.; Vialta,Airton; Keid,Lara Borges; Dias,Ricardo Augusto; Genovez,Margareth E.. |
| A total of 192 samples of illegal cheese from different regions of the states of São Paulo and Minas Gerais, Brazil, were analyzed for the isolation and detection of Brucella spp. DNA by means of microbiological culture and polymerase chain reaction (PCR), respectively. Samples that yielded positive results were submitted to the analysis of the occurrence of Brucella abortus (biovars 1, 2 e 4), as well as to the differentiation of DNA in B19 vaccinal strain or Brucella abortus field strain using PCR. Although the microorganism was not isolated from any sample, PCR detected 37 positive samples (19.27%) using genus-specific primers. From these, all (100%) were Brucella abortus. Differentiation of the strain showed that 30/37 samples (81.08%) were vaccinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella abortus; B19; PCR; Cheese. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100005 |
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| Scarcelli,Eliana; Piatti,Rosa Maria; Fedullo,José Daniel Luzes; Simon,Faiçal; Cardoso,Maristela Vasconcellos; Castro,Vanessa; Miyashiro,Simone; Genovez,Margareth Élide. |
| Leptospirosis is a widely distributed zoonosis that affects domestic and wild animals, and that has the man as the end point of its epidemiological chain. Leptospirosis diagnosis in primates is more difficult than in other animal species, as clinical signs and lesions are less evident and antibody response is detected only for short periods. The aim of this article was to describe the detection of Leptospira spp using polymerase chain reaction (PCR), in clinical samples from one captive black-capped Capuchin monkey (Cebus apella), which presented characteristics compatible with leptospirosis (jaundice and haemorrhagic kdney) in the macroscopic post-mortem examination. A friable kidney fragment and urine sample were cultured and submitted to experimental... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Nonhuman primate; PCR; Cebus apella. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200010 |
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| Genovez,Margareth Élide; Yasuda,Paulo Hideki; Vasconcellos,Silvio Arruda; Scarcelli,Eliana; Cardoso,Maristela Vasconcellos; Girio,Raul da Silva. |
| The Counterimmunoelectrophoresis reaction (CIE) was tested as a genus specific test for swine leptospirosis diagnosis using three soluble extracts obtained from Leptospira sp, serovars pomona, icterohaemorrhagiae and patoc. The extracts were treated with hot Triton X-100 and applied to serum samples of animals divided in three groups: Group 1, 10 swines experimentally infected with the Pomona strain; Group 2, 50 naturally infected swines and Group 3, 10 swines control. These animals were serologically evaluated by CIE and by the Microscopic Agglutination Test (MAT), the WHO reference technique. Groups 1 and 3 were monitored during a period of 93 days after infection (a.i.). Group 1 serum convertion took place around the 10th day a.i. by MAT but ocurred... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Counterimmunoelectrophoresis; Swine leptospirosis; Seradiagnosis. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200016 |
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| Scarcelli,Eliana; Costa,Elizabeth Oliveira da; Genovez,Margareth Élide; Cardoso,Maristela Vasconcellos; Bach,Erna Elizabeth; Torres,Ana Paula. |
| Electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni strains isolated from feces of seven animal species, including man, were compared. Fourteen strains (two from each species) plus two human strains and the reference one, were ruptured by ultrasound and their total soluble proteins were analyzed by SDS-PAGE technique in a 12% polyacrylamide gel with computerized densitometric reading by the molecular analyst software. All the strains had bands in common that correspond to 45 and 66 Kda molecular weight. The disagreement corresponded to a 97 to 200 Kda molecular weight region. From the 17 strains, 13 (76.5%), were classified as biotype I, three (17.6%) as biotype II and one (5.8%) as biotype III. Since protein extracts were obtained from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; SDS-PAGE; Biotyping. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400006 |
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| Carvalho,Aline Feola de; Silva,Daniela Martins da; Azevedo,Sergio Santos; Piatti,Rosa Maria; Genovez,Margareth Elide; Scarcelli,Eliana. |
| Campylobacteriosis is a worldwide distributed zoonosis. One of the main virulence factors related to Campylobacter spp. in animals and humans is the cytolethal distending toxin (CDT), encoded by three adjacent genes (cdtA, cdtB, cdtC). The occurrence of Campylobacter spp. in samples of vegetables has not been reported in Brazil yet, and has seldom been described in the international literature. The detection of CDT in these strains has not been reported, either. The objectives of the present study were to determine the occurrence of Campylobacter spp. strains carrying virulence factors in samples of poultry and vegetables (lettuce and spinach) from different points of sale, thus verifying if vegetables are as an important vehicle for potentially virulent... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter spp.; Cytolethal distending toxin (CDT); Broiler carcasses; Vegetables; Multiplex-PCR. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300005 |
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