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| Registros recuperados: 13 | |
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| Lima, J. Y. O.; Achkar, S. M.; Scheffer, K. C.; Castilho, J. G.; Rodrigues, A. C.; Kotait, I.; Carrieri, M. L.. |
| Bats are considered important reservoirs of rabies virus, which is paramount in the study of the pathogenesis of this zoonosis disease, through the use of sensitive systems. The presence of viral antigen in these species shows that non-neuronal viral spread is efficient in different organs that participate effectively in the elimination of rabies virus, such as, salivary glands and bladder. This study aimed to investigate the presence of rabies virus in samples of bats submitted for laboratory diagnosis, as well as, to study the pathogenesis of the disease through the use of laboratory animals. 3,930 routine diagnostic specimens of bats were processed during the period between January 2011 and May 2012 by direct immunofluorescence (DIF) and viral isolation... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3078 |
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| Scheffer, K. C.; Di Gregorio, M. C.; Batista, A. M.; Rosato, P. N.; Campos Filho, E.; Silva, A. C. R.; Caporale, G. M. M.; Cruz, P. S.; Chaves, L. B.. |
| Among the factors that establish the pre‐analytical variability, some studies report that hemolysis is the main interferential in several tests. However, no studies were found on immunofluorescence tests or that using cell cultures. The aim of this study was to evaluate the interference of different degrees of hemolysis serum samples in the evaluation of virus neutralizing antibodies (VNA) by rapid fluorescent focus inhibition test (RFFIT). We obtained 27 blood samples from dogs vaccinated for rabies. Hemolysis was caused by mechanical induction and freezing. Hemolysated sera were classified visually and by measurement of hemoglobin made by spectrophotometry. The evaluation of VNA in the samples was performed by RFFIT. The results were statistically... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17379 |
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| Freitas, A. P.; Peixoto, Z. M. P.; Silva, A. C. R.; Scheffer, K. C.; Chaves, L. B.; Caporale, G. M. M.. |
| The fluorescent anti-rabies conjugate (FARC) produced in Instituto Pasteur de São Paulo (IP-SP) is obtained by fluorescein isothiocyanate (FITC) conjugation with specific polyclonal antibodies purified and it recommended storage temperature of 4°C protected from light incidence. Given the fundamental importance of this reagent for rabies laboratorial diagnosis, the aim of this study was evaluate its stability in relation to physical factors that can affect the quality and maintenance of product characteristics. To do this, different aliquots of FARC batch IP-TOT1/11 submitted to temperatures of 37, 56, 65 and 80°C for 1hour or exposed to ambient light for 10, 20, 30, 45, 60, 90, 120, 150 minutes and 24 hours were used on direct immunefluorescence (DIF)... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3071 |
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| Scheffer, K. C.; Fahl, W. O.; Iamamoto, K.; Carnieli Jr., P.; Carrieri, M. L.; Oliveira, R. N.; Ito, F. H.. |
| Molecular techniques have been used increasingly as tools for the diagnosis by detecting the rabies virus genome. This study aimed to detect the presence of rabies virus in the wash of skull and in different organs of the genus Artibeus bats using the hemi-nested RT-PCR (hnRT-PCR) and Real Time RT-PCR molecular techniques. From approximately 4,000 specimens of bats received at the Institute Pasteur for rabies diagnosis, 30 bats of the genus Artibeus were selected, with records of positive results for rabies by the traditional techniques of direct fluorescent antibody test (FAT) and inoculation of murine neuroblastoma cell line (N2A). Salivary glands, urinary bladders, kidneys, lungs, and also the washes of the skullcaps of the specimens were collected. The... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3105 |
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| Ikeda, M. A. K.; Barros, R. F.; Lima, J. Y. O.; Ferreira, J. S.; Pereira, P. M. C.; Fahl, W. O.; Iamamoto, K.; Mori, E.; Ferreira, E.; Achkar, S. M.; Scheffer, K. C.. |
| The bats are a major reservoir of rabies virus and have a relevant importance in the disease transmission. The aim of this study was to evaluate the bat population submitted for rabies diagnosis. Data were analyzed from 18.805 bats received by the diagnostic section of the Pasteur Institute of Sao Paulo, originated from several counties of the state of Sao Paulo over the period from 2007 to 2012. These specimens were morphologically classified according to their family, at the time of execution of the rabies diagnosis technique. The central nervous system from these animals was submitted to direct immunofluorescence test and, when viable, to viral isolation. From the total of bats received, 76.92% bats belonged to the Molossidae family, 12.14% to the... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17383 |
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| Mori, E.; Scheffer, K. C.; Fahl, W. O.; Oliveira, R. N.; Asano, K. M.; Carnieli Jr, P.; Carrieri, M. L.; Kotait, I.. |
| Human rabies is still an important public health problem in some Brazilian regions. Usually, ante-mortem diagnosis of rabies is made by demonstration of virus antigen by direct immunofluorescence in corneal or conjunctival smears and skin biopsies; however, this technique has a low sensitivity. Recently, molecular techniques such as the reverse transcriptase polymerase chain reaction (RT-PCR) and nucleic acid sequence based amplification assay (NASBA) have been developed to improve the sensitivity and specificity of ante as well as post-mortem diagnosis of rabies. Rapid and accurate diagnosis of ante-mortem human rabies is essential for effective medical management and to ensure appropriate post-exposure prophylaxis of potential contacts with the patient.... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3098 |
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| Pereira, P. M. C.; Lima, J. Y. O.; Barros, R. F.; Ferreira, J. S.; Ikeda, M. A. K.; Fahl, W. O.; Iamamoto, K.; Mori, E.; Asano, K. M.; Ferreira, E.; Achkar, S. M.; Scheffer, K. C.. |
| Bats found in Brazil are classified in 9 families, 64 genus and 168 species. They are an important rabies reservoirs and all species can be infected or be the transmitter of rabies virus, independently of their specie classification or feeding habits. This work aimed a survey of bats species originated from counties of Sao Paulo state, which were sent to the diagnostic laboratory of the Pasteur Institute – Sao Paulo. Between August 2009 to October 2012; 9.109 bats specimens were received and submitted to recommended techniques for rabies diagnosis by World Health Organization. From these specimens, 8.421 (92,45%) were classified in family, genus and specie, by morphological and morphometric parameters using bats identification keys. Furthermore, they also... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17386 |
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| Ferreira, J. S.; Trotti, A. C. P.; Scheffer, K. C.; Caporale, G. M. M.; Silva, A. C. R.; Chaves, L. B.. |
| The Fluorescent Antibody Virus Neutralization Test (FAVN) is used routinely in many laboratories for the reference measurement of rabies virus neutralizing antibodies (VNA) in serum of animals to confirm the efficacy of vaccination against rabies, which is required to authorize the transit of these animals in countries free of rabies. The World Organization for Animal Health (OIE) recommends a VNA title ≥ 0,5 IU/mL to ensure that the animal has immunity against the rabies virus. The rabies laboratory diagnosis of Pasteur Institute of São Paulo/Brazil (IP/SP) is a national reference of the Ministry of Health for rabies diagnosis and performs the measurement of VNA in humans and animals serum samples for proof of immunization against rabies. The objective of... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/2526 |
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| Silva, V. A.; Gamom, T. H. M.; Silva, A. C. R.; Caporale, G. M. M.; Chaves, L. B.; Scheffer, K. C.. |
| O principal objetivo da vacinação do Programa Nacional de Profilaxia da Raiva é manter índices imunogênicos protetores nos animais vacinados, esperando-se que os títulos de anticorpos neutralizantes (AcN) sejam ≥ 0,5UI/mL. Tanto a Organização Mundial da Saúde (OMS) como a Oficina Internacional de Epizootias (OIE) consideram essa titulação como referência de status de proteção contra o vírus da raiva e um indicador para avaliar a eficácia da vacina. O objetivo deste estudo foi avaliar, de acordo com a idade, raça e o período entre a aplicação da vacina e a colheita do sangue, a resposta imunológica de cães primo vacinados com vacina de cultivo celular. Para avaliar a resposta imune em cães foram analisadas 432 amostras recebidas no Instituto Pasteur de São... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/16813 |
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| Scheffer, K. C.; Fahl, W. O.; Lamamoto, K.; Carnieli Junior, P.; Carrieri, M. L.; Mori, E.; Oliveira, R. N.; Ito, F. H.. |
| The aim of this study was to detect the rabies virus (RABV) presence in different organs of frugivorous bats using molecular techniques such as RT‐PCR, hnRT‐PCR, and the Real Time RT‐PCR. Thirty bats of the genus Artibeus were selected and resulted as positive by the DFA test and N2A‐cells inoculation test using brain tissue in both tests. Samples of salivary gland tissue, urinary bladder tissue, kidney tissue, lung tissue, stool, and skull lavage were collected for molecular assays. The organs and the stool were diluted at 1:10 (w/v) and the urinary bladder was diluted at 1:20 (w/v). The RT‐PCR and the hnRT‐PCR were performed using specific nucleoprotein gene‐target primers. The product obtained by reverse transcription technique was submitted to the Real... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17385 |
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| Freitas, A. P.; Silva, A. C. R.; Scheffer, K. C.; Pimenta, D. C.. |
| Rabies is an infectious disease caused by a virus belonging to the family Lissavirus that affects all mammals including humans. Rabies transmission occurs by virus-containing saliva , through biting or licking of an injured tissue. The virus replicates at the site of inoculation until it reaches the nerve endings. At the neuromuscular junctions, the virus binds specifically to the nicotinic acetylcholine receptor. On the other hand, alkaloids have yielded interesting results in terms of drug development. Since rabies virus infection is, at least, partially associated to the nicotinic acetylcholine receptor, the present study focus on the evaluation of molecules obtained from the skin secretion of Ameerega picta, in the process of penetration and infection... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/2570 |
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| Gamon, T. H. M.; Silva, V. A.; Silva, A. C.R.; Caporale, G. M. M.; Chaves, L. B.; Centoamore, N. H. F.; Souza, T. C. P.; Scheffer, K. C.. |
| The World Health Organization (WHO) and the Office International desEpizooties (OIE) consider as being the reference status of protection againstrabies titers of virus neutralizing antibodies (VNA) = 0.5 IU/mL to maintaina protective immune response in animals. The aim of this study was to evaluate,according to age, race and period of vaccination and blood collection,the immune response in dogs prime vaccinated with rabies cell culture vaccine.Based on request forms, 432 samples of animals received at the PasteurInstitute of Sao Paulo at the period of 2009 to 2011, those receiving a singledose of vaccine by the time of blood collection were analyzed. We evaluatedthe information on age, race and period of vaccination until to blood collection.The evaluation... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/22172 |
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| Lima, J. Y. O.; Scheffer, K. C.; Achkar, S. M.; Kotait, I.; Carrieri, M. L.. |
| The diversity of bat species in Brazil is great, and there are 172 species distributed among nine families. The Phyllostomidae family is the most numerous, followed by Vespertilionidae and Molossidae. According to feeding habits, the majority of bats are insectivorous, followed by frugivorous, nectarivorous, carnivorous and hematophagous. As these animals are considered reservoirs of rabies virus it is essential to correctly identify the species and knowledge of the c rmv s p . g o v . b r mv&z 79 biology, behavior and adaptation of bats in urban environments. The objective of this study was to identify the several specimens of bats received for rabies diagnosis at the Pasteur Institute, meet the positive index of them and assess their distribution on... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3079 |
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| Registros recuperados: 13 | |
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