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	Provedor de dados BJM (2) Genet. Mol. Biol. (1) Autor Schrank,Irene Silveira (3) Alves-Junior,Leonardo (1) Botton,Sônia de Avila (1) Brocchi,Marcelo (1) Cantarelli,Vlademir Vicente (1) Carvalho,Marcos Oliveira de (1) Costa,Mateus Matiuzzi da (1) Drescher,Guilherme (1) Maboni,Franciele (1) Pereira,Fabiana de Souza (1) Pinto,Paulo Marcos (1) Ruppenthal,Rubia Denise (1) Vainstein,Marilene Henning (1) Vargas,Agueda Castagna de (1) Weber,Shana (1) Mais... Palavra-chave Antimicrobial resistance (1) Constins (1) LGT (1) Molecular diagnosis (1) Mycoplasma (1) Neonatal (1) PCR (1) Plasmids (1) Sepsis (1) Swine (1) Virulence factors (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2008 (1) 2007 (1) 2005 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae Genet. Mol. Biol. Pinto,Paulo Marcos; Carvalho,Marcos Oliveira de; Alves-Junior,Leonardo; Brocchi,Marcelo; Schrank,Irene Silveira. Diversification of bacterial species and pathotypes is largely caused by lateral gene transfer (LGT) of diverse mobile DNA elements such as plasmids, phages, transposons and genomic islands. Thus, acquisition of new phenotypes by LGT is very important for bacterial evolution and relationship with hosts. This paper reports a 23 kb region containing fourteen CDSs with similarity to the previous described Integrative Conjugal Element of Mycoplasma fermentans (ICEF). This element, named ICEH, is present as one copy at distinct integration sites in the chromosome of 7448 and 232 pathogenic strains and is absent in the type strain J (non-pathogenic). Notable differences in the nucleotide composition of the insertion sites were detected, and could be correlated... Tipo: Info:eu-repo/semantics/article Palavras-chave: LGT; Mycoplasma; Constins. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200014 Application of broad-range bacterial PCR amplification and direct sequencing on the diagnosis of neonatal sepsis BJM Ruppenthal,Rubia Denise; Pereira,Fabiana de Souza; Cantarelli,Vlademir Vicente; Schrank,Irene Silveira. A broad-range bacterial PCR target to conserved regions of the 23S rDNA was applied to 306 blood culture samples from 295 infants (up to one year of age) admitted to a neonatal intensive care unit. Classic blood culture results were compared to DNA sequencing analysis of the PCR amplification products. Culture results were in agreement to DNA sequencing in 90.5% (277) of 306 samples tested, including 263 PCR and culture negative samples and 29 culture and PCR positive samples. The sensitivity of the PCR method combined with sequencing was 88%, and the specificity was 96.3%, with positive and negative predictive values of 74.3 e 98.5%, respectively. The PCR-based approach directly applied to blood culture samples, correlated well with blood culture results... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sepsis; Molecular diagnosis; Neonatal; PCR. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100006 Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil BJM Costa,Mateus Matiuzzi da; Drescher,Guilherme; Maboni,Franciele; Weber,Shana; Botton,Sônia de Avila; Vainstein,Marilene Henning; Schrank,Irene Silveira; Vargas,Agueda Castagna de. The present study determined the molecular and resistance patterns of E. coli isolates from urinary tract of swine in Southern of Brazil. Molecular characterization of urinary vesicle samples was performed by PCR detection of virulence factors from ETEC, STEC and UPEC. From a total of 82 E. coli isolates, 34 (38.63%) harbored one or more virulence factors. The frequency of virulence factors genes detected by PCR were: pap (10.97%), hlyA (10.97%), iha (9.75%), lt (8.53%), sta (7.31%) sfa (6.09%), f4 (4.87%), f5 (4.87%), stb (4.87%), f6 (1.21%) and f41 (1.21%). Isolates were resistant to penicillin (95.12%), lincomycin (93.9%), erythromycin (92.68%), tetracycline (90.24%), amoxicillin (82.92%), ampicillin (74.39%), josamycin (79.26%), norfloxacin (58.53%),... Tipo: Info:eu-repo/semantics/article Palavras-chave: Plasmids; Virulence factors; Antimicrobial resistance; Swine. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400027 Registros recuperados: 3 Primeira ... 1 ... Última

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