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Schuck,Mariane Ruzza; Biasi,Luiz Antonio; Mariano,Ada Michele; Lipski,Bernardo; Riaz,Summaira; Walker,Michael Andrew. |
The objective of this work was to assess the potential of interspecific hybridization of Vitis labruscana and Muscadinia rotundifolia by using artificial cross-pollinations. Microsatellite markers were used to confirm interspecific hybridizations and the identity of the parental genotypes. In crosses in which M. rotundifolia was used as the female parent, no true hybrids were obtained. In the reciprocal crosses, 114 seedlings were identified as true V. labruscana x M. rotundifolia hybrids. Self pollination occurred in direct and in reciprocal crosses. The crossings between 'Bordo' x 'Carlos', 'Magnolia', 'Regale' and' Roanoke', and between' Isabel' x 'Bountiful', 'Carlos', 'Magnolia', 'Regale' and 'Roanoke' were confirmed. The 15 markers evaluated showed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Muscadinia rotundifolia; Vitis labruscana; Hybrid identification; Interspecific crosses; SSRs. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001100009 |
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Schuck,Mariane Ruzza; Moreira,Flavia Maia; Guerra,Miguel Pedro; Voltolini,José Afonso; Grando,Maria Stella; Silva,Aparecido Lima da. |
The objective of this work was to characterize the grape germplasm in Santa Catarina, Brazil, using microsatellite DNA markers (simple sequence repeats - SSR). The DNA samples were collected from leaves and shoots of accessions of public and private collections from the counties Urussanga, Nova Trento, Rodeio, São Joaquim, Campos Novos, Videira, and Água Doce. Ten SSR loci (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79, VVMD25, VVMD28, VVMD31, and VVMD32) were analysed by capillary electrophoresis. Molecular profiling was conducted for 190 grapevines (European, American, and hybrids), and 67 genotypes were obtained. The data were compared with each other and with those from the literature and from online databases, in order to identify varieties and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitis spp.; Cultivar identification; Genetic variability; Germplasm collection; Molecular markers. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000500008 |
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Schuck,Mariane Ruzza; Moreira,Flavia Maia; Voltolini,José Afonso; Guerra,Miguel Pedro; Grando,Maria Stella; Silva,Aparecido Lima da. |
A variedade Goethe é símbolo da vitivinicultura da região de Urussanga, sul do Estado de Santa Catarina, a qual, atualmente, busca a Indicação Geográfica da Uva e do Vinho Goethe. Para isto, um dos requisitos necessários é a identificação precisa do material genético. Os marcadores microssatélites constituem a ferramenta molecular mais utilizada para a identificação varietal de videira em todo o mundo e têm a capacidade de produzir um perfil genético único para cada material vitícola. O objetivo deste trabalho foi caracterizar duas seleções de uva 'Goethe', presentes no município de Urussanga, por meio de marcadores moleculares microssatélites, visando a atender aos requisitos de denominação de origem e indicação de procedência controlada. A extração do... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitis; Identificação varietal; Marcadores microssatélites; Variabilidade intravarietal. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000300023 |
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Carvalho,Dayse Cristina de; Silva,André Luís Lopes da; Schuck,Mariane Ruzza; Purcino,Marivel; Tanno,Guilherme Nakao; Biasi,Luiz Antonio. |
The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine) levels on in vitro shoot regeneration of fox grape cv. Bordô and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15, 30 and 45 g.L-1 were tested as a carbon source and the supplementation of adenine, asparagine, alanine, glycine, cysteine, glutamine, arginine was tested at 40 g.L-1. The BAP levels (1 and 5 μM) in solid and double-phase media were evaluated and compared with a control medium without BAP. Bordô had best in vitro growth than Chardonnay. Sucrose was a better carbohydrate source than glucose for both the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arginine; Nodal segment; Glucose; Adenine; Glycine. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200004 |
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