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Microsatellite molecular marker-assisted gene pyramiding for resistance to Asian soybean rust (ASR) Agronomy
Viganó, Joselaine; Braccini, Alessandro Lucca; Schuster, Ivan; Menezes, Vanessa Maria Pereira Silva.
The present study aimed at pyramiding ASR-resistance genes through microsatellite (SSR) marker-assisted selection (MAS) and demonstrating the pyramiding steps. To obtain the first generation of gene pyramiding, crosses were made between introduced plants (PI’s), which have the genes Rpp1, Rpp2, Rpp3, Rpp4, and Rpp5. F1 plants from the initial crosses were intercrossed to obtain plants with the four resistance genes (second pyramiding generation). Plants selected from this second generation were again intercrossed (third pyramiding generation) to increase the number of pyramided genes. For MAS, we used informative SSR markers in each cross. SSR markers were considered informative when the source resistance allele containing the target gene could be followed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phakopsora pachyrhizi; Gene stacking; Marker-assisted breeding; Durable resistance..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39619
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Microsatellite molecular marker-assisted gene pyramiding for resistance to Asian soybean rust (ASR) Agronomy
Viganó, Joselaine; Braccini, Alessandro Lucca; Schuster, Ivan; Menezes, Vanessa Maria Pereira Silva.
The present study aimed at pyramiding ASR-resistance genes through microsatellite (SSR) marker-assisted selection (MAS) and demonstrating the pyramiding steps. To obtain the first generation of gene pyramiding, crosses were made between introduced plants (PI’s), which have the genes Rpp1, Rpp2, Rpp3, Rpp4, and Rpp5. F1 plants from the initial crosses were intercrossed to obtain plants with the four resistance genes (second pyramiding generation). Plants selected from this second generation were again intercrossed (third pyramiding generation) to increase the number of pyramided genes. For MAS, we used informative SSR markers in each cross. SSR markers were considered informative when the source resistance allele containing the target gene could be followed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phakopsora pachyrhizi; Gene stacking; Marker-assisted breeding; Durable resistance..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39619
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Mapping and validation of molecular markers of genes Dt1 and Dt2 to determine the type of stem growth in soybean Agronomy
Vicente, Dorival; Schuster, Ivan; Lazzari, Fabiane; Paranzini, Jonas Pedro Dolfini; Oliveira, Marco Antonio Rott de; Prete, Cássio Egídio Cavenaghi.
The type of stem growth in soybean is a distinguishing feature of cultivars. The genes Dt1 and Dt2 affect the termination of the stem, and the types of growth are classified as determinate, semi-determinate and indeterminate. Phenotypic characterization of the type of growth is complex and is occasionally erroneously described. The objective of this study was to map and validate molecular markers to classify the type of soybean growth to facilitate the description of cultivars and genotypic selection. Two populations were used for mapping and validation of molecular markers F2:3: T 117 (semi-determinate growth type) x Igra RA 518 RR (indeterminate growth type) and CD 235RR (determinate growth type) x Igra RA 518 RR. The study demonstrates that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Determinate growth; Semi-determinate growth; Indeterminate growth; SAM.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/26216
Registros recuperados: 3
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