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Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis BJM
Mirhosseini,Seyed Ali; Fooladi,Abbas Ali Imani; Amani,Jafar; Sedighian,Hamid.
ABSTRACT Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have been developed. In this study, recombinant FliC (rFliC) was produced to be used as an antigen. The immunization was conducted in mice with the purified recombinant FliC (rFliC). The mice were subcutaneously immunized with rFliC and elicited significant rFliC specific serum IgG antibodies. An...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella Enteritidis; Flagellin C; Indirect ELISA; Bacterial detection; Food contaminated.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400774
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