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| Monte,Leonardo Garcia; Jorge,Sérgio; Luiz,João Paulo Mesquita; Sinnott,Francine; Seixas,Fabiana Kömmling; Aleixo,José Antonio Guimarães; Samartino,Luis Ernesto; Conceição,Fabricio Rochedo; Hartleben,Cláudia Pinho. |
| Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine leptospirosis; IMS-PCR; LipL32. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200023 |
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| Miraglia,Fabiana; Moreno,Andréa Mike; Gomes,Cleise Ribeiro; Paixão,Renata; Liuson,Esequiel; Morais,Zenaide Maria; Maiorka,Paulo; Seixas,Fabiana Kömmling; Dellagostin,Odir Antonio; Vasconcellos,Silvio Arruda. |
| With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers > 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pomona; Swine; VNTR; Culture; PCR; Genital Tract. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017 |
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| Nassi,Fernanda; Seixas,Fabiana Kömmling; Jouglard,Sandra Denize Dorneles; Simionatto,Simone; Silva,Everton Fagonde; Seyffert,Núbia; Brod,Claudiomar Soares; Dellagostin,Odir Antonio. |
| Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods. The aim of this work was to evaluate and optimize a Nested-PCR method for diagnosis of leptospirosis. Primers were designed to amplify a 264 bp region within the LipL32 gene. The sensitivity and specificity of the assay was evaluated using seven saprophytic serovars and 35 pathogenic serovars. This technique showed to be very specific for pathogenic serovars, however it lacked sensitivity. In order to enhance the sensitivity,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500031 |
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