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| Castro,André L. M. de; Vollú,Renata E.; Peixoto,Raquel S.; Grigorevski-Lima,André L.; Coelho,Rosalie R. R.; Bon,Elba P. S.; Rosado,Alexandre S.; Seldin,Lucy. |
| A cellulolytic bacterial strain, designated P118, isolated from the gut of the tropical fish Parotocinclus maculicauda was identified as belonging to the genus Paenibacillus based on phenotypic and chemotaxonomic characteristics and the 16S rRNA gene sequence. The novel strain was Gram-positive, spore-forming and rod-shaped. Catalase but not oxidase was produced. Carboxymethylcellulose was hydrolyzed but starch or gelatin was not. Acetoin production was negative whereas nitrate reduction and urease production were positive. Many carbohydrates served as carbon sources for growth. MK-7 was the predominant isoprenoid quinone. Anteiso-C15:0 (38.73%) and C16:0 (20.85%) were the dominant cellular fatty acids. Strain P118 was closely related to Paenibacillus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioprospection; Cellulose degradation; Paenibacillus sp.; Parotocinclus maculicauda; Taxonomy. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400048 |
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| Santos,Anderson F.; Valle,Roberta S.; Pacheco,Clarissa A.; Alvarez,Vanessa M.; Seldin,Lucy; Santos,André L.S.. |
| Halophilic microorganisms are source of potential hydrolytic enzymes to be used in industrial and/or biotechnological processes. In the present study, we have investigated the ability of the moderately halophilic bacterium Halobacillus blutaparonensis (strain M9), a novel species described by our group, to release proteolytic enzymes. This bacterial strain abundantly proliferated in Luria-Bertani broth supplemented with 2.5% NaCl as well as secreted proteases to the extracellular environment. The production of proteases occurred in bacterial cells grown under different concentration of salt, ranging from 0.5% to 10% NaCl, in a similar way. The proteases secreted by H. blutaparonensis presented the following properties: (i) molecular masses ranging from 30... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Halobacillus blutaparonensis; Halophilic bacterium; Serine protease. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400039 |
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| Raposeiras,Rui; Pinto,Patrícia P.; Passos,Raul V.M.; Seldin,Lucy; Paiva,Edilson; Scotti,M. Rita; Sá,Nadja M.H.. |
| Irregular response to bean plants to Rhizobium inoculation has been attributed to among other factors, low competitive ability, low N2 fixation efficiency and genetic instability of the symbiont. This genetic instability caused by high rates of genomic rearrangements and/or plasmid deletions can be accentuated by high temperatures. This fact may limit the utilization of these strains as inoculants, especially in tropical soils. In this study, the variability of isolated colonies derived from effective R. leguminosarum bv. phaseoli (SLP1.3 and BR 10.026) and R tropici (SLA2.2 and BR322) strains was evaluated before and after exposure to high temperatures (four consecutive thermal shocks at 45ºC). This evaluation involved plant dry matter analysis of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhizobium tropici; Rhizobium leguminosarum bv. phaseoli; Bean plants; High temperature; Cerrado soils. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200010 |
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| Lima,André Suêldo Tavares de; Barreto,Maria do Carmo Silva; Araújo,Janete Magali; Seldin,Lucy; Burity,Hélio Almeida; Figueiredo,Márcia do Vale Barreto. |
| O feijão-caupi Vigna unguiculata [L.] Walp. é a principal cultura de subsistência do semiárido brasileiro, sendo fonte de proteínas de baixo custo, notadamente, para populações carentes. A produção dessa cultura no Nordeste é baixa devido à não utilização de insumos agrícolas - entre eles, o fertilizante nitrogenado. Por outro lado, bactérias promotoras de crescimento em plantas (BPCPs) vêm sendo estudadas, de forma a maximizar a fixação de N2, disponibilizar nutrientes como P ou fito-hormônios e inibir doenças. Os objetivos deste trabalho foram verificar a viabilidade da coinoculação das sementes de feijão-caupi usando Paenibacillus, Brevibacillus e, ou, Bacillus e Bradyrhizobium-caupi; caracterizar as estirpes quanto à produção de ácido indol acético... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Promoção de crescimento; Solubilização de fosfato; Vigna unguiculata; AIA; Nodulação. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000300006 |
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| Pinto,Patrícia P.; Raposeiras,Ruy; Macedo,Andrea M.; Seldin,Lucy; Paiva,Edilson; Sá,Nadja M.H.. |
| High temperatures can affect the survival, establishment and symbiotic properties of Rhizobium strains. Bean nodulating Rhizobium strains are considered particularly sensitive because on this strains genetic recombinations and/or deletions occur frequently, thus compromising the use of these bacteria as inoculants. In this study R. tropici and R. leguminosarum bv. phaseoli strains isolated from Cerrado soils were exposed to thermal stress and the strains growth, survival and symbiotic relationships as well as alterations in their genotypic and phenotypic characteristics were analyzed. After successive thermal shocks at 45ºC for four hours, survival capacity appeared to be strain-specific, independent of thermo-tolerance and was more apparent in R. tropici... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bean nodulating Rhizobium strains; High temperatures. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400012 |
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