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	Provedor de dados BJM (1) Electron. J. Biotechnol. (1) Autor Sette,Lara Durães (2) Macedo,Juliana Alves (1) Oliveira,Valéria Maia de (1) Santos Neto,Eugênio Vaz dos (1) Sato,Hélia Harumi (1) Simioni,Karen Christina Marques (1) Mais... Palavra-chave 16S rDNA (1) Bacterial diversity (1) Central composite design (1) Community DNA (1) Cultivation-independent analysis (1) Fractional factorial design (1) Medium optimization (1) Microbial transglutaminase (1) Petroleum (1) Streptomyces sp. P20 (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2008 (1) 2007 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Optimization of medium composition for transglutaminase production by a Brazilian soil Streptomyces sp. Electron. J. Biotechnol. Macedo,Juliana Alves; Sette,Lara Durães; Sato,Hélia Harumi. Finding a new microbial source of transglutaminase (MTGase) and the study of the medium composition for MTGase production were the goals of this work. A total of 200 actinomycete-like strains were isolated from Brazilian soil samples and two of them named T10b and P20 were selected based on their ability to produce 0.15 U.mL-1 and 0.25 U.mL-1 of MTGase, respectively. Strain P20 was chosen to continue the study and was identified as Streptomyces sp. In order to optimize the MTGase activity, modifications of the usual media composition described for enzyme production were tested. The strategy adopted was: (1) screening experiment for the best carbon and nitrogen sources; (2) fractional factorial design (FFD) to elucidate the key ingredients in the media (the... Tipo: Journal article Palavras-chave: Central composite design; Fractional factorial design; Medium optimization; Microbial transglutaminase; Streptomyces sp. P20. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400014 Bacterial diversity characterization in petroleum samples from Brazilian reservoirs BJM Oliveira,Valéria Maia de; Sette,Lara Durães; Simioni,Karen Christina Marques; Santos Neto,Eugênio Vaz dos. This study aimed at evaluating potential differences among the bacterial communities from formation water and oil samples originated from biodegraded and non-biodegraded Brazilian petroleum reservoirs by using a PCR-DGGE based approach. Environmental DNA was isolated and used in PCR reactions with bacterial primers, followed by separation of 16S rDNA fragments in the DGGE. PCR products were also cloned and sequenced, aiming at the taxonomic affiliation of the community members. The fingerprints obtained allowed the direct comparison among the bacterial communities from oil samples presenting distinct degrees of biodegradation, as well as between the communities of formation water and oil sample from the non-biodegraded reservoir. Very similar DGGE band... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Community DNA; Cultivation-independent analysis; Petroleum; 16S rDNA. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300007 Registros recuperados: 2 Primeira ... 1 ... Última

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