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| Nassi,Fernanda; Seixas,Fabiana Kömmling; Jouglard,Sandra Denize Dorneles; Simionatto,Simone; Silva,Everton Fagonde; Seyffert,Núbia; Brod,Claudiomar Soares; Dellagostin,Odir Antonio. |
| Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods. The aim of this work was to evaluate and optimize a Nested-PCR method for diagnosis of leptospirosis. Primers were designed to amplify a 264 bp region within the LipL32 gene. The sensitivity and specificity of the assay was evaluated using seven saprophytic serovars and 35 pathogenic serovars. This technique showed to be very specific for pathogenic serovars, however it lacked sensitivity. In order to enhance the sensitivity,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500031 |
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| Silva,Éverton F.; Seyffert,Núbia; Cerqueira,Gustavo M.; Leihs,Karl P.; Athanazio,Daniel A.; Valente,Ana L. S.; Dellagostin,Odir A.; Brod,Claudiomar S.. |
| In this study, we observed the presence of antileptospiral agglutinins in freshwater turtles of two urban lakes of Pelotas, Southern Brazil. Forty animals (29 Trachemys dorbigny and 11 Phrynops hilarii) were captured and studied. Attempts to isolate leptospires from blood and urine samples were unsuccessful. Serum samples (titer > 100) reactive to pathogenic strains were observed in 11 animals. These data encourage surveys of pet turtles to evaluate the risk of transmission of pathogenic leptospires to humans. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Turtles; Agglutinins. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200003 |
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| Seyffert,Núbia; Silva,Éverton Fagonde da; Bermudez,Viviane Lorenzato; Brod,Claudiomar Soares. |
| In leptospirosis, the Microscopic Agglutination Test (MAT) is the basis of serological diagnosis and the most widely used. In our study, the MAT was applied to evaluate the influence of temperature, incubation time, antigen density and age of the culture in the agglutinability of Leptospires and the interference of these factors in diagnosis. Three serum samples with titers of 100, 800 and 25.600 to the serovar tande (local isolate) of canicola serogroup were used with different combinations among the four factors. There was a significant relation among all factors, specially between the antigen density and age of the culture. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Microscopic agglutination; Leptospirosis; Laboratorial diagnosis. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500025 |
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