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	Provedor de dados Electron. J. Biotechnol. (1) Autor Arjmand,Sareh (1) Lotfi,Abbas Sahebghadam (1) Mowla,Seyed Javad (1) Shamsara,Mehdi (1) Palavra-chave Human alpha 1-antitrypsin (1) Pichia pastoris (1) Recombinant proteins (1) Tipo do documento Journal article (1) Ano 2013 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Elevating the expression level of biologically active recombinant human alpha 1-antitrypsin in Pichia pastoris Electron. J. Biotechnol. Arjmand,Sareh; Lotfi,Abbas Sahebghadam; Shamsara,Mehdi; Mowla,Seyed Javad. Background: Human alpha 1-antitrypsin (AAT) is a potent inhibitor of multiple serine proteases, and protects tissues against their harmful effects. Individuals with reduced or abnormal production of this inhibitor need intravenous administration of exogenous protein. In this study, we employed the methylotrophic (methanol utilizing) yeast Pichia pastoris (P. pastoris) as a preferential host for efficient production and secretion of recombinant AAT. Furthermore, we examined different strategies to maximize the yield of the secreted protein. Results: Our findings revealed that optimizing the codon usage of AAT gene for P. pastoris had positive effects on the level of secreted AAT under the control of inducible alcohol oxidase 1 (AOX1) and constitutive... Tipo: Journal article Palavras-chave: Human alpha 1-antitrypsin; Pichia pastoris; Recombinant proteins. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100004 Registros recuperados: 1 Primeira ... 1 ... Última

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