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	Provedor de dados Electron. J. Biotechnol. (2) Autor Kumar,Vinod (2) Sharma,Ashwani (2) Aswathanarayana Ravishankar,Gokare (1) Bhaskar Gururaj,Harishchandra (1) Giridhar,Parvatam (1) Narasimha Prasad,Bellur Chayapathy (1) Ravishankar,Gokare Aswathanarayana (1) Mais... Palavra-chave Agrobacterium rhizogenes (1) Capsicum frutescens (1) Cobalt chloride (1) Hairy roots (1) In vitro flowering (1) Nicotiana tabacum (1) Pollen transformation (1) Regeneration (1) Silver nitrate (1) Transformation frequency (1) Ultra-sonication (1) Mais... Tipo do documento Journal article (2) Ano 2008 (1) 2006 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation Electron. J. Biotechnol. Sharma,Ashwani; Kumar,Vinod; Giridhar,Parvatam; Ravishankar,Gokare Aswathanarayana. The influence of silver nitrate (AgNO3) and cobalt chloride (CoCl2) on shoot multiplication and in vitro flowering in Capsicum frutescens Mill. was investigated. Exogenous administration of AgNO3 and CoCl2 at a concentration of 30 µM resulted in the maximum tissue response in terms of shoot length and number of shoots after 45 days culturing on MS medium. Both silver nitrate (40 µM) and cobalt chloride (30 µM) influenced in vitro flowering after 25 and 45 days respectively. This is the first report on in vitro flowering in C. frutescens. The study also demonstrated successful transformation of pollen obtained from the in vitro flowers. Since capsicum is highly recalcitrant to in vitro plant regeneration, the results of the study may be highly useful in... Tipo: Journal article Palavras-chave: Capsicum frutescens; Cobalt chloride; In vitro flowering; Pollen transformation; Regeneration; Silver nitrate. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200011 Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment Electron. J. Biotechnol. Kumar,Vinod; Sharma,Ashwani; Narasimha Prasad,Bellur Chayapathy; Bhaskar Gururaj,Harishchandra; Aswathanarayana Ravishankar,Gokare. The phytopathogenic bacteria Agrobacterium rhizogenes genetically transforms plants by transferring a portion of the resident Ri plasmid, the T-DNA to the plant. Plant species differ in their temporal competence for transformation. But various physical and chemical methods are found to enhance transformation frequency. Agrobacterium rhizogenes mediated transformation efficiency was assessed under the influence of sonication, calcium treatment, acetosyringone and macerating enzymes in suitable combinations in Nicotiana tabacum as a model system. Manual wounding resulted in 21% transformation frequency. Where as sonication resulted in 2.2 fold increase, followed by sonication with CaCl2 treatment resulted in 2.5 fold increase and sonication with... Tipo: Journal article Palavras-chave: Agrobacterium rhizogenes; Hairy roots; Nicotiana tabacum; Transformation frequency; Ultra-sonication. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400002 Registros recuperados: 2 Primeira ... 1 ... Última

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