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| Hirumi, Hiroyuki; Hirumi, K.; Moloo, S. K.; Shaw, M. K.. |
| Bloodstream trypomastigotes (BSFs) of 2 clones (IL 1392 and IL 3671) of Trypanosoma vivax were cultured without feeder layers in 3 systems. System I: metacyclic-producing cultures of T. vivax IL 1392 were initiated with BSFs derived from infected mice at 27 OC in the presence of feeder layers using TVM-1 medium. Metacyclics harvested were then transferred to flasks containing feeder cells and maintained at 34 OC using TVM-22 medium. Under such conditions, the metacyclics transformed to BSFs. Bloodstream trypomastigotes which were then transferred to new flasks, continued to grow in HMI-162 medium without feeder cells. The maximum density of the BSFs and their shortest population doubling time were 3.5 x l06 / ml and 13.5 h, respectively. System II:... |
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Palavras-chave: Feeder cell layers; In vitro cultivation; Trypomastigotes. |
| Ano: 1991 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/153 |
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