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Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom J. Venom. Anim. Toxins incl. Trop. Dis.
Shirmardi,SP; Shamsaei,M; Gandomkar,M; Saniei,E; Ghannadi,M; Zare,A.
Iranian scorpions belong mainly to the Buthidae and Scorpionidae families, distributed into 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming; amongst which Mesobuthus eupeus is the most common. Its venom contains several toxin fractions that may affect the ion channel. In the present study purification, labeling and biological evaluation of M. eupeus venom are described. For separation, soluble venom was loaded on a chromatography column packed with Sephadex G-50 gel. Subsequently, the fractions were collected according to UV absorption at a wavelength of 280 nm. Toxic fraction (F3) was loaded on an anionic ion exchanger resin and then on a cationic resin....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesobuthus eupeus; Venom; Purification; Chromatography; Radiolabeling; Biodistribution.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400015
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