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| Shu,Yongjun; Li,Wei; Zhao,Jinyue; Zhang,Sijia; Xu,Hanyun; Liu,Ying; Guo,Changhong. |
| Abstract Alfalfa (Medicago sativa L.) is an important perennial forage, with high nutritional value, which is widely grown in the world. Because of low freezing tolerance, its distribution and production are threatened and limited by winter weather. To understand the complex regulation mechanisms of freezing tolerance in alfalfa, we performed transcriptome sequencing analysis under cold (4 °C) and freezing (-8 °C) stresses. More than 66 million reads were generated, and we identified 5767 transcripts differentially expressed in response to cold and/or freezing stresses. These results showed that these genes were mainly classified as response to stress, transcription regulation, hormone signaling pathway, antioxidant, nodule morphogenesis, etc., implying... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Medicago sativa L.; RNA-seq; Freezing tolerance; C-repeat binding factors (CBF). |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500824 |
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| Li,Wei; Liu,Ying; Zhao,Jinyue; Zhen,Xin; Guo,Changhong; Shu,Yongjun. |
| Abstract MYB is a large family of plant transcription factors. Its function has been identified in several plants, while there are few reports in Medicago truncatula. In this study, we used RNA-seq data to analyze and identify R2R3-MYB genes in the genome of Medicago truncatula. Phylogenetic analysis classified 150 MtMYB genes into 21 subfamilies with homologs. Out of the 150 MtMYB genes, 139 were distributed among 8 chromosomes, with tandem duplications (TD) and segment duplications (SD). Microarray data were used for functional analysis of the MtMYB genes during growth and developmental processes providing evidence for a role in tissues differentiation, seed development processes, and especially the nodulation process. Furthermore, we investigated the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Medicago truncatula; MYB; Abiotic stress; QRT-PCR. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400611 |
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| Shu,Yongjun; Li,Wei; Zhao,Jinyue; Liu,Ying; Guo,Changhong. |
| Abstract Medicago ruthenica is a perennial forage legume with the remarkable ability to survive under unfavorable environmental conditions. It has been identified as an excellent species of Medicago that can adapt to various environmental stresses including low temperature, drought, and salinity. To investigate its potential as a genetic resource, we performed transcriptome sequencing and analysis in M. ruthenica under abiotic stresses. We generated >120 million reads from six cDNA libraries, resulting in 79,249 unique transcripts, most of which were highly similar to transcripts from M. truncatula (44,608, 56.3%) and alfalfa (M. sativa, 48,023, 60.6%). Based on gene expression profiles, 2,721 transcripts were identified as abiotic stress responsive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Medicago ruthenica; RNA-Seq; Transcription factor (TF); Abiotic stress; Reactive oxygen species (ROS). |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400638 |
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