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Molecular characterization of papaya genotypes using AFLP markers Rev. Bras. Frutic.
Oliveira,Eder Jorge de; Costa,Juliana Leles; Santos,Lucas Ferraz dos; Carvalho,Fabiana Moraes de; Silva,Aline dos Santos; Dantas,Jorge Luiz Loyola.
Due to the low genetic variability reported in the commercial plantations of papaya (Carica papaya L.), the objective of this study was analyze the genetic diversity of 32 genotypes including cultivars, landraces, inbred lines, and improved germplasm using the AFLP technique (Amplified Fragment Length Polymorphism). The genetic distance matrix was obtained using the Nei and Li genetic distance and clustering was performed using the unweighted pair-method with arithmetic mean (UPGMA). Using 11 combinations of EcoRI/MseI primers, 383 polymorphic bands were obtained. On average, 34.8 polymorphic bands were obtained per primer combination. Five clusters were formed. The traditional cultivar 'Sunrise' and the inbred line CMF-L30-08 were the closest genotypes,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Papaya breeding; Genetic resources; Genetic diversity; Carica papaya L.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000300020
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Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers Scientia Agricola
Silva,Aline dos Santos; Oliveira,Eder Jorge de; Haddad,Fernando; Jesus,Onildo Nunes de; Oliveira,Saulo Alves Santos de; Costa,Maria Angélica Pereira de Carvalho.
Fusarium oxysporum f. sp. passiflorae W.L. Gordon (FOP) is one of the most important fungal pathogens of passion fruits. Understanding molecular variation of isolates from different areas is of utmost importance. Molecular fingerprinting on 14 isolates of FOP were conducted using AFLP molecular markers (Amplified Fragment Length Polymorphism), and their genetic variability were estimated. Twenty-five AFLP primer combinations were selected for amplification of FOP isolates and one for Fusarium oxysporum f. sp. cubense W.C. Snyder & H.N. Hansen (FOC), resulting in 99% polymorphic fragments, with an average of 40 fragments per primer combination. Specific fingerprints could be generated for most of the isolates evaluated; we observed a high power of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passion fruit; Fusarium wilt; Fingerprint; Variability; Molecular marker.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000200008
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