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	Provedor de dados Anais da ABC (AABC) (1) Autor Bertani,Giovani R. (1) Gil,Laura H.V.G. (1) Marques Jr,Ernesto T.A. (1) Queiroz,Sabrina R.A. (1) Santos,Jefferson J.S. (1) Silva,Andréa N.M.R. (1) Mais... Palavra-chave Cloning technique (1) Homologous recombination (1) Replicon (1) Reporter gene (1) Yellow fever virus (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2013 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Construction of yellow fever virus subgenomic replicons by yeast-based homologous recombination cloning technique Anais da ABC (AABC) Queiroz,Sabrina R.A.; Silva,Andréa N.M.R.; Santos,Jefferson J.S.; Marques Jr,Ernesto T.A.; Bertani,Giovani R.; Gil,Laura H.V.G.. RNA replicon derived from Flavivirus genome is a valuable tool for studying viral replication independent of virion assembly and maturation, besides being a great potencial for heterologous gene expression. In this study we described the construction of subgenomic replicons of yellow fever virus by yeast-based homologous recombination technique. The plasmid containing the yellow fever 17D strain replicon (pBSC-repYFV-17D), previously characterized, was handled to heterologous expression of the green fluorescent protein (repYFV-17D-GFP) and firefly luciferase (repYFV-17D-Luc) reporter genes. Both replicons were constructed by homologous recombination between the linearized vector pBSC-repYFV-17D and the PCR product containing homologous 25 nucleotides ends... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cloning technique; Homologous recombination; Replicon; Reporter gene; Yellow fever virus. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100159 Registros recuperados: 1 Primeira ... 1 ... Última

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