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Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients BJID
Ferreira,Thiago de Oliveira; Koto,Rafael Yoshio; Leite,Gabriel Fialkovitz da Costa; Klautau,Giselle Burlamaqui; Nigro,Stanley; Silva,Cely Barreto da; Souza,Ana Paula Idalgo da Fonseca; Mimica,Marcelo Jenne; Cesar,Regina Grigolli; Salles,Mauro José Costa.
Abstract Objectives To compare cultured microorganisms identified on endotracheal tubes biofilms through sonication technique with traditional tracheal aspirate collected at extubation of pediatric intensive care unit patients. Methods Demographic and epidemiological data were analyzed to identify factors possibly related with the microbiological profile of the two collection methods. Associations between categorical and continuous variables were analyzed using the chi-square or Fisher's exact test, or Student's t test. p-Value <0.05 were considered significant. Results Thirty endotracheal tubes and tracheal aspirates samples from 27 subjects were analyzed. Only one patient presented the clinical diagnosis of ventilator-associated pneumonia. Overall,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sonication; Endotracheal tube; Biofilm; Tracheal aspirate.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000500468
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Evaluation of the in vitro antimicrobial activity of an ethanol extract of Brazilian classified propolis on strains of Staphylococcus aureus BJM
Pamplona-Zomenhan,Lucila Coelho; Pamplona,Beatriz Coelho; Silva,Cely Barreto da; Marcucci,Maria Cristina; Mimica,Lycia Mara Jenné.
Staphylococcus aureus (S. aureus) is one of the most frequent causes of hospital acquired infections. With the increase in multiple drug resistant strains, natural products such as propolis are a stratagem for new product discovery. The aims of this study were: to determine the in vitro antimicrobial activity of an ethanol extract of propolis; to define the MIC50 and MIC90 (Minimal Inhibitory Concentration - MIC) against 210 strains of S. aureus; to characterize a crude sample of propolis and the respective ethanol extract as to the presence of predetermined chemical markers. The agar dilution method was used to define the MIC and the high performance liquid chromatography (HPLC) method was used to characterize the samples of propolis. MIC results ranged...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Staphylococcus aureus; Antimicrobial Activity.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400002
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