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Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method BABT
Parizzi,Silvânia Quintão Fontes; Andrade,Nélio José de; Silva,Cleuber Antonio de Sá; Soares,Nilda de Fátima Ferreira; Silva,Eldo Antônio Monteiro da.
The adherence of Listeria inoccua L6a and Staphylococcus aureus ATCC6538 was evaluated on stainless steel (SS), polycarbonate (PC) and polypropylene (PP) chips. The EP results showed a significant difference (p<0.05) among the microorganism species interactions, the surfaces and the contact time. The number of adhered cells on the chip surfaces increased as contact time increased and the number of L. innocua or S. aureus on the surface of SS, PC and PP chips reached 1.0x10(5) CFU/cm² after 12 h of contact, in both methods. The CP method showed a better sensitivity to detect low number of adhered cells. The EP was better when the average number of adhered cells was between 10 and 100 per microscopy field.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial adherence; Listeria innocua and Staphylococcus aureus; Stainless steel; Polycarbonate and polypropylene.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011
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Evaluation of ultraviolet radiation to control microorganisms adhering to low-density polyethylene films BJM
Silva,Cleuber Antonio de Sá; Andrade,Nélio José de; Soares,Nilda de Fátima Ferreira; Ferreira,Sukarno Olavo.
Efficiency of ultraviolet (UV) radiation in reducing the cell number of Staphylococcus aureus ATCC 25923 and Escherichia coli K-12 adhered to low-density polyethylene (LDPE) films was evaluated. The microorganisms were let to adhere to the surface of LPDE bags for 12h at 18ºC, and then submitted to UV radiation at an intensity 196 µW.cm-2, 254nm, for 2 seconds. Staphylococcus aureus was less resistant to UV radiation than E. coli, and the efficiency increased with the increase of the concentration of microbial suspension. After 1500 hours of use the UV radiation intensity of the lamp was reduced from 288 to 78 µW.cm-2, and the higher decrease occurred in the first 100 hours of use. Also, the efficiency of the UV radiation decreased after 1500 hours of use....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial adhesion; Ultraviolet radiation; Low-density polyethylene; Atomic force microscopy.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200017
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