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The use of standard methodology for determination of antifungal activity of natural products against medical yeasts Candida sp and Cryptococcus sp BJM
Scorzoni,Liliana; Benaducci,Tatiane; Almeida,Ana Marisa Fusco; Silva,Dulce Helena Siqueira; Bolzani,Vanderlan da Silva; Gianinni,Maria José Soares Mendes.
Methodology for testing natural compounds for determination of antifungal activity had been developed with adaptations. The most used are bioautography and agar diffusion with a complex and no defined media. In this study, different methods for determination of antifungal activity of natural products are discussed and the use of M27-A2 microdilution test from CLSI (Clinical and Laboratory Standards Institute, 2002), as general standard methodology for testing plant extracts activity is recommended.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Natural products; Antifungal activity; Candida sp; Cryptococcus sp.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300001
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Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator BJM
Lisboa,Helen Cristina Fávero; Biasetto,Carolina Rabal; Medeiros,João Batista de; Araújo,Ângela Regina; Silva,Dulce Helena Siqueira; Teles,Helder Lopes; Trevisan,Henrique Celso.
A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project "Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest". The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi (EC. 3.1.1.1 -carboxylesterases) for production of carboxylic acids. Thus, the reduction of the pH increases...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Esterase; PH indicator; Enzyme activity; Endophytic fungi.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300042
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