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	Provedor de dados BJM (1) Autor Bellei,N.C.J. (1) Carraro,E. (1) Granato,C.F.H. (1) Perosa,A.H.S. (1) Silva,E.R.M. (1) Watanabe,A.S.A. (1) Mais... Palavra-chave Detection (1) Differentiation (1) Enterovirus (1) Rhinovirus (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2012 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Rhinovirus detection using different PCR-based strategies BJM Silva,E.R.M.; Watanabe,A.S.A.; Carraro,E.; Perosa,A.H.S.; Granato,C.F.H.; Bellei,N.C.J.. Human rhinoviruses (HRVs) are the major cause of the common cold. HRVs were recently reclassified into the Enterovirus genus (HEV) in the Picornaviridae family. HRVs and other members of the HEV genus share many common features, including sense RNA genomes and partial nucleotide sequence identity. The aim of this study was to evaluate different HRV detection strategies. Samples from adults with acute respiratory infection (n = 291) who were treated in Sao Paulo Hospital (2001-2003) were tested using three assays. The first assay detected picornaviruses by RT-PCR and hybridization, the second detected rhinoviruses using RT-PCR/sequencing, and the third differentiated HRV from HEV using duplex semi-nested-RT-PCR. Analysis of the results obtained from the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhinovirus; Enterovirus; Detection; Differentiation. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200038 Registros recuperados: 1 Primeira ... 1 ... Última

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