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Utilization of fluorescent probe association for simultaneous assessment of plasmatic, acrosomal, and mitochondrial membranes of rooster spermatozoa Rev. Bras. Ciênc. Avic.
Celeghini,ECC; Arruda,RP; Albuquerque,R; Silva,FHA; Faria,DE; Andrade,AFC; Nascimento,J; Raphael,CF.
This experiment was designed with the objective of developing a simple, practical, and high repeatability technique for the simultaneous evaluation of the integrity of the plasmatic and acrosomal membranes, as well as funcional mitochondria of domestic fowl spermatozoa using an association of fluorescent probes. Four ejaculates (motility > 80% and abnormal morphology < 10%) from each of six Ross male broiler breeder (n=24) were diluted in TALP sperm medium (25x10(6) spermatozoa/mL) and split into two aliquots, and one of these aliquots was flash frozen in liquid nitrogen and thawed to damage all cellular membranes. Three treatments were prepared from these aliquots, with the following ratios of Fresh semen:Flash frozen semen: 100:0 (T100), 50:50...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fluorescent probes; Membranes; Rooster; Sperm.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000300001
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Influence of egg pre-storage heating period and storage length on the digestive tract of newly-hatched broiler chicks Rev. Bras. Ciênc. Avic.
Silva,FHA; Faria,DE; Torres,KAA; Faria Filho,DE; Coelho,AAD; Savino,VJM.
An experiment was carried out to evaluate the effect of different heating times of settable eggs of Cobb 500® broiler breeders before submitting them to different storage periods on body weight, digestive tract organ weights, and intestinal mucosa morphology of newly-hatched chicks. Settable eggs were distributed in a completely randomized experimental design with a 3 x 3 factorial arrangement: pre-storage heating periods (0, 6, 12 hours at 36.92°C) and storage periods (4, 9, 14 days at 12.06°C). Body weight and relative weights of the yolk sac, heart, liver, proventriculus+gizzard, and intestinal segments were measured in chicks hatching at 480 and 498 hours of incubation. Villi height, width and perimeter, and crypt depth (ìm) were measured in duodenal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broilers; Embryo development; Incubation; Intestinal mucosa; Morphometrics.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2008000100004
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Influence of egg pre-storage heating period and storage length on incubation results Rev. Bras. Ciênc. Avic.
Silva,FHA; Faria,DE; Torres,KAA; Faria Filho,DE; Coelho,AAD; Savino,VJM.
This experiment aimed at evaluating the influence of different heating times of settable eggs of Cobb 500® broiler breeders before submitting them to different storage periods on egg weight loss, embryo mortality, and hatchability. A total number of 1,980 eggs were distributed in a completely randomized experimental design with a 3 x 3 factorial arrangement, comprising nine treatments with 22 replicates of 10 eggs each. The following factors were analyzed: pre-storage heating periods (0, 6, 12 hours at 36.92°C) and storage periods (4, 9, 14 days at 12.06°C). After storage, eggs were incubated under usual conditions, and were transferred to the hatcher at 442 hours of incubation. Eggs were weighed before heating, incubation, and transference to determine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler breeders; Egg weight loss; Embryo development; Embryo mortality; Hatch.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2008000100003
Registros recuperados: 3
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