|
|
|
|
|
| Souza,Samara Silva de; Silva,Grazyelle Sebrenski da; Almeida-Val,Vera Maria Fonseca de. |
| Abstract The present study aimed to evaluate the biological responses of Colossoma macropomum to naphthalene injection and subsequent hypoxia exposure, emphasizing the expression of the tumor suppressor gene tp53. Tambaquis were intraperitoneally injected with naphthalene (50 mg/kg) and, after 96 hours, the fish were transferred to respirometry chambers and, submitted to progressive hypoxia for the determination of critical PO2. In a subsequent experiment, the fish received an intraperitoneal injection of naphthalene and were kept for 96 hours under normoxia. Successively, fish were challenged with acute hypoxia (PO2<PO2 crit) during 6 hours. We observed that the PO2 crit was not affected by naphthalene injection. Moreover, hematological parameters were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Naphthalene; Hypoxia; Tumor suppressor gene tp53; DNA damage; Tambaqui. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300411 |
| |
|
|
| Silva,Grazyelle Sebrenski da; Fé,Luciana Mara Lopes; Silva,Maria de Nazaré Paula da; Val,Vera Maria Fonseca de Almeida e. |
| Abstract Benzo[a]pyrene (B[a]P) is a petroleum derivative capable of inducing cancer in human and animals. In this work, under laboratory conditions, we analyzed the responses of Colossoma macropomum to B[a]P acute exposure through intraperitoneal injection of four different B[a]P concentrations (4, 8, 16 and 32 μmol/kg) or corn oil (control group). We analyzed expression of the ras oncogene and the Hypoxia-inducible factor-1 alpha (hif-1α) gene using quantitative real-time PCR. Additionally, liver histopathological changes and genotoxic effects were evaluated through the comet assay. Ras oncogene was overexpressed in fish exposed to 4, 8 of 16 μmol/kg B[a]P, showing 4.96, 7.10 and 6.78-fold increases, respectively. Overexpression also occurred in hif-1α... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ras oncogene; Hif-1α; Tambaqui; B[a]P; Genotoxic damage. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300491 |
| |
|
|
|
