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Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species Rev. Bras. Ciênc. Solo
Florentino,Ligiane Aparecida; Sousa,Pedro Martins de; Silva,Jacqueline Savana; Silva,Karina Barroso; Moreira,Fatima Maria de Souza.
The genetic diversity of ten Bradyrhizobium strains was evaluated for tolerance to high temperatures, to different salinity levels and for the efficiency of symbiosis with cowpea plants (Vigna unguiculata (L.) Walp.). Eight of these strains were isolated from nodules that appeared on cowpea after inoculation with suspensions of soil sampled from around the root system of Sesbania virgata (wand riverhemp) in ecosystems of South Minas Gerais. The other two strains used in our analyses as references, were from the Amazon and are currently recommended as cowpea inoculants. Genetic diversity was analyzed by amplifying repetitive DNA elements with the BOX primer, revealing high genetic diversity with each strain presenting a unique band profile. Leonard jar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological N2 fixation; Selection; Inoculant strains; Phenotypic diversity; Genotypic diversity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000400011
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Symbiotic nitrogen-fixing bacterial populations trapped from soils under agroforestry systems in the Western Amazon Scientia Agricola
Jaramillo,Paula Marcela Duque; Guimarães,Amanda Azarias; Florentino,Ligiane Aparecida; Silva,Karina Barroso; Nóbrega,Rafaela Simão Abrahão; Moreira,Fatima Maria de Souza.
Cowpea (Vigna unguiculata) is an important grain-producing legume that can forego nitrogen fertilization by establishing an efficient symbiosis with nitrogen-fixing bacteria. Although inoculating strains have already been selected for this species, little is known about the genotypic and symbiotic diversity of native rhizobia. Recently, Bradyrhizobium has been shown to be the genus most frequently trapped by cowpea in agricultural soils of the Amazon region. We investigated the genetic and symbiotic diversity of 148 bacterial strains with different phenotypic and cultural properties isolated from the nodules of the trap species cowpea, which was inoculated with samples from soils under agroforestry systems from the western Amazon. Sixty non-nodulating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Legume-nodulating bacteria; Biodiversity; Symbiotic promiscuity; Chlorophyll content.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000600004
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Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species Scientia Agricola
Florentino,Ligiane Aparecida; Jaramillo,Paula Marcela Duque; Silva,Karina Barroso; Silva,Jacqueline Savana da; Oliveira,Silvia Maria de; Moreira,Fatima Maria de Souza.
Biological nitrogen fixation, performed by diazotrophic bacteria, plays an important role in the maintenance of agricultural systems, as it contributes with significant amounts of the nitrogen (N) needed for plant growth, totally or partially exempting the use of industrial N fertilisers. Twenty-five bacterial strains isolated from nodules of Leucaena leucocephala and Phaseolus vulgaris trap plants were studied. These nodules were formed after inoculation with suspensions of soil samples collected close to the root system of Sesbania virgata. In previous studies, these bacteria were identified as Cupriavidus necator. This study aimed to evaluate the ability of these strains to fix N2 in the free-living state and to use carbon (C) sources; their resistance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Symbiosis; Β-proteobacteria; Antibiotic resistance.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400003
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