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Comparison between different dilution rates on canine semen freezing using Tris-buffer with the addition of egg-yolk and glycerol Arq. Bras. Med. Vet. Zootec.
Silva,A.R.; Cardoso,R.C.S.; Silva,L.D.M..
Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10(6) spermatozoa/ml and the second was a volume:volume extension at a proportion of one part semen to one part extender. Semen was frozen, stored in liquid nitrogen and thawed after one week. Sperm motility and vigor were appraised after each stage of the process and at 15 and 30min post-thawing. Sperm morphology was analyzed after collection and thawing. No differences were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Semen; Extender; Freezing; Dilution.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600010
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Relação entre perímetro escrotal e concentração espermática em cães, clinicamente normais, da raça Pastor Alemão Arq. Bras. Med. Vet. Zootec.
Cortez,A.A.; Aquino-Cortez,A.; Silva,A.R.; Cardoso,R. C.S.; Silva,L.D.M..
The aim of this study was to estimate the correlation between scrotal girth and spermatic concentration in German Sheepherd dogs. Thirteen German Sheepherd dogs were used and 44 semen samples were obtained by digital manipulation. The ejaculate was separated in its three fractions. The sperm rich fraction was kept for evaluation and determination of spermatic concentration by spectrofotometry. The parameters were expressed as mean and standard deviation. Spearman correlation test was used to estimate the correlation between spermatic concentration and scrotal girth. The mean spermatic concentration was 568.45±314.91x10(6) sptz/ml and the mean scrotal girth was17.6±1.6 cm and the correlation was r = 0.10. It can be conclude that scrotal girth can not be an...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cão; Sêmen; Concentração espermática; Perímetro escrotal.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000500017
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Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles Arq. Bras. Med. Vet. Zootec.
Domingues,S.F.S.; Ferreira,H.S.; Muniz,J.A.P.C.; Lima,A.K.F.; Ohashi,O.M.; Figueiredo,J.R.; Silva,L.D.M..
The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monkey; Preantral follicle; Isolation; Ovary; Cebus apella.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009
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Recovery of sperm after epididymal refrigeration from domestic cats using ACP-117c and Tris extenders Arq. Bras. Med. Vet. Zootec.
Lima,D.B.C.; Silva,T.F.P.; Cortez,A. Aquino; Pinto,J.N.; Magalhães,F.F.; Caldini,B.N.; Silva,L.D.M..
ABSTRACT We aimed to compare fresh sperm and sperm cooled to 4ºC that had been recovered from the epididymides of cats using powdered coconut water (ACP-117c) and Tris extenders. Sixty epididymides were divided into 6 groups: 10 fresh epididymides were recovered using Tris (T0h); 10 were kept at 4°C/2h and recovered using Tris (T2h); 10 were kept at 4°C/4h and recovered using Tris (T4h); 10 fresh were recovered using ACP-117c (A0h); 10 were kept at 4°C/2h and recovered using ACP-117c (A2h), and 10 were kept at 4°C/4h and recovered using ACP-117c (A4h). The testis-epididymis complexes (TEC) control were not cooled. The others were cooled at 4°C for 2 or 4h. The epididymis was separated and the sperm was recovered by the modified flotation method. Sperm...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cat; Epididymal spermatozoa; Cooling; Powdered coconut water.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000400873
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