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Comparison of three laboratorial tests for diagnosis of canine parvovirus infection Arq. Bras. Med. Vet. Zootec.
Silva,M.M.O.; Castro,T.X.; Costa,E.M.; Trancoso,T.A.L.; Mendes-de-Almeida,F.; Labarthe,N.V.; Cubel Garcia,R.C.N..
The aim of this study was to evaluate the rapid tests currently used for canine parvovirus (CPV) diagnosis: hemagglutination test (HA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). A total of 112 fecal samples collected from diarrheic puppies up to one year of age were tested. The EIA was able to detect CPV antigen in 44 samples. By HA, 32 samples tested highly positive with titers &gt;128, eight tested weakly positive (titers 32 and 64) and 72 were negative (titers <16). Using PCR, 57 samples were found positive including 13 EIA-negative and 19 HA-negative samples. The best correlation was observed between EIA and PCR (88.4%). These tests were able to detect all types of CPV, including CPV-2c. Considering that 23%-33% of dogs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine parvovirus; Enteritis; HA; EIA; PCR.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100023
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