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Comparison of two slide agglutination serotyping methods and PCR-based capsule typing for the characterization of Haemophilus influenzae serotypes BJM
Silva,Maria E.N. Bonifácio da; Silva,Paulo da; Medeiros,Marta I.C.; Neme,Suzel N.; Macedo,Claudia; Marin,José Moacir.
Ninety-three nasopharyngeal Haemophilus influenzae isolates were serotyped by two slide agglutination methods (SAST 1 and SAST 2) and the results compared with those obtained by capsule type-specific PCR. SAST 1 presented a low correlation with results obtained by PCR (75.2%) while SAST 2 showed a better agreement with the molecular technique results (93.5%). These findings suggest that SAST 2 could be an alternative method for adequate detection of H.influenzae type b.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Haemophilus influenzae; Serotyping methods; PCR.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100007
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Nasopharyngeal colonization by Haemophilus influenzae in children attending day-care centers, in Ribeirão Preto, State of São Paulo, Brazil BJM
Silva,Maria E.N. Bonifácio da; Silva,Paulo da; Medeiros,Marta I.C.; Neme,Suzel N.; Macedo,Claudia; Marin,José Moacir.
Nasopharyngeal carriage of Haemophilus influenzae (Hi) was studied in 114 healthy children < 3 years old, attending day-care centers (DCCs) in Ribeirão Preto, State of São Paulo, Brazil. Biotype, serotype (by specific antisera and PCR) and antibiotic susceptibility to 14 antibiotics of each isolate were determined. Carriage rates of Hi were 72.0%. Isolates belonged to biotype II (36.5%), I (21.5%), V (18.2%) and III (16.1%). The prevalence of encapsulated Hi carriers was 3.2% for type f, 1.0% for type b, 1.0% for type d and 1.0% for type e. Resistances to trimethoprim-sulphamethoxazole and ampicillin were 46.2% and 10.7% respectively. Multidrug resistance was found in 14 (15.0%) of the isolates tested. Among the isolates, 13.9% were beta-lactamase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haemophilus influenzae; Day-care center; Hib colonization; Hib vaccination.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100006
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