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	Provedor de dados BABT (1) BJPS (1) Autor Silva,Mauro Ramalho (2) Silva,Viviane Dias Medeiros (2) Silvestre,Marialice Pinto Coelho (2) Afonso,Wendel de Oliveira (1) Lopes Junior,Carlos de Oliveira (1) Morais,Harriman Aley (1) Silva,Ana Cristina Simões e (1) Silveira,Josianne Nicácio (1) Souza,Mariana Wanessa Santana de (1) Mais... Palavra-chave Angiotensin-converting enzyme (1) Aspergillus oryzae Protease (1) Degree of Hydrolysis (1) Inhibitory activity (1) Pancreatin (1) Peptide profile (1) Peptides (1) Protein Hydrolysates (1) Protein hydrolysates (1) Whey (1) Whey proteins (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2012 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Action of a pancreatin and an Aspergillus oryzae protease on whey proteins: correlation among the methods of analysis of the enzymatic hydrolysates BABT Morais,Harriman Aley; Silvestre,Marialice Pinto Coelho; Silveira,Josianne Nicácio; Silva,Ana Cristina Simões e; Silva,Viviane Dias Medeiros; Silva,Mauro Ramalho. The objectives of this study were to hydrolyze whey proteins using a pancreatin and an Aspergillus oryzae protease; to evaluate the degree of hydrolysis (DH) and the peptide profile; and to establish the correlations among the analytical methods. Ten hydrolysates were prepared at different reaction times and the highest DH was obtained by the protein content method. Good correlations (r > 0.87) between the methods of formaldehyde and orthophthalaldehyde (OPA), formaldehyde and osmometry as well as osmometry and OPA were observed using pancreatin. Similar results were obtained between OPA and soluble protein content for the A. oryzae protease. The action of pancreatin produced the highest contents of di- and tripeptides (9.07, 7.12 and 6.46%) and the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Whey; Protein Hydrolysates; Degree of Hydrolysis; Peptides; Pancreatin; Aspergillus oryzae Protease. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600014 Analysis of whey protein hydrolysates: peptide profile and ACE inhibitory activity BJPS Silvestre,Marialice Pinto Coelho; Silva,Mauro Ramalho; Silva,Viviane Dias Medeiros; Souza,Mariana Wanessa Santana de; Lopes Junior,Carlos de Oliveira; Afonso,Wendel de Oliveira. The aim of this study was to prepare enzymatic hydrolysates from whey protein concentrate with a nutritionally adequate peptide profile and the ability to inhibit angiotensin-converting enzyme (ACE) activity. The effects of the type of enzyme used (pancreatin or papain), the enzyme:substrate ratio (E:S ratio=0.5:100, 1:100, 2:100 and 3:100) and the use of ultrafiltration (UF) were investigated. The fractionation of peptides was performed by size-exclusion-HPLC, and the quantification of the components of the chromatographic fractions was carried out by a rapid Corrected Fraction Area method. The ACE inhibitory activity (ACE-IA) was determined by Reverse Phase-HPLC. All parameters tested affected both the peptide profile and the ACE-IA. The best peptide... Tipo: Info:eu-repo/semantics/article Palavras-chave: Whey proteins; Protein hydrolysates; Peptide profile; Inhibitory activity; Angiotensin-converting enzyme. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400019 Registros recuperados: 2 Primeira ... 1 ... Última

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