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	Provedor de dados BABT (2) Autor Higuti,Ilma Hiroko (2) Nascimento,Aguinaldo José do (2) Silva,Priscila Anunciação da (2) Andrade,Edicléia Alves de (1) Marcondes,Aluizio de Abreu (1) Okiyama,Vivian Mayumi de Eiróz (1) Papp,Juliana (1) Mais... Palavra-chave Bacillus firmus (2) Cyclodextrin glycosyltransferase (2) Cyclodextrins (1) Starch (1) Tipo do documento Info:eu-repo/semantics/article (2) Ano 2004 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Colorimetric determination of alpha and beta-cyclodextrins and studies on optimization of CGTase production from B. firmus using factorial designs BABT Higuti,Ilma Hiroko; Silva,Priscila Anunciação da; Papp,Juliana; Okiyama,Vivian Mayumi de Eiróz; Andrade,Edicléia Alves de; Marcondes,Aluizio de Abreu; Nascimento,Aguinaldo José do. Cyclodextrin glycosyltransferase (EC 2.4.1.19, CGTase) production from B. firmus, isolated from soil of Curitiba, PR, was optimized in shake flask using an experimental design approach. The CGTase was produced when the carbon source was starch and beta-CD, but when simple sugars such as glucose, galactose, lactose, sucrose, and maltose were used, there was no enzyme production. CGTase production was the same with either organic nitrogen or inorganic nitrogen source. CGTase activity decreased 2-fold when incubation temperature was increased from 28 to 37 ° C, and decreased 2.1- fold when the initial pH was lowered from 10.3 to 7.4. The colorimetric determinations of alpha - and beta -CD were analyzed as a non-linear relationship and the equilibrium constant... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Cyclodextrins. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600001 Studies on alkalophilic CGTase-producing bacteria and effect of starch on cyclodextrin-glycosyltransferase activity BABT Higuti,Ilma Hiroko; Silva,Priscila Anunciação da; Nascimento,Aguinaldo José do. The production of beta- and gamma -CD by CGTase from Bacillus firmus was studied, in regard to the effect of the source and concentration of starch on the yield of CD and CGTase activity. A cyclic activity of accumulation and consumption of beta -CD and gamma -CD occurred during the bacterial growth. CGTase was more active when citrate buffer, pH 5.5 was used. No differences were found for production of gamma -CD with the use of commercial starch flour when compared with corn starch (p>0.05). The use of commercial starch flour resulted in decreased conversion of starch to beta-CD (all the 4 starch sources were statistically different, p<0.05). The best results for beta -CD production were obtained with the use of corn starch. The specific activity of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Starch. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100018 Registros recuperados: 2 Primeira ... 1 ... Última

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