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| Fonseca,Isabela; Silva,Priscila Vendramini; Lange,Carla Christine; Guimarães,Marta F.M.; Weller,Mayara Morena Del Cambre Amaral; Sousa,Katiene Régia Silva; Lopes,Paulo Sávio; Guimarães,José Domingos; Guimarães,Simone E.F.. |
| In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytokine; Gene expression; Immune response; Real-time PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400018 |
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| Silva,Kleibe de Moraes; Paixão,Débora Martins; Silva,Priscila Vendramini; Solero,Bruna Pena; Pereira,Mario Sérgio; Lopes,Paulo Sávio; Guimarães,Simone Eliza Facioni. |
| The objective was to map QTL on porcine chromosome 4 and to associate them with carcass and internal organ traits in an F2 population. The F1 population was produced by outbreed crossing, using two native Brazilian breed Piau boars and 18 commercial sows. A total of 617 F2 animals issued from 11 F1 boars and 54 F1 sows were typed for a total of five microsatellite markers. The data were analyzed by multiple regressions developed for the analysis of crosses between outbred lines, using the QTL Express software. Significant evidence for QTL was found for pig chromosome 4 regarding carcass and internal organ traits. All QTL were detected in the same region of the chromosome, designated FAT1. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fatness; Pig production; Divergent crosses; Microsatellite markers; QTL mapping. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300013 |
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| Faria,Danielle Assis de; Peixoto,Jane de Oliveira; Lopes,Paulo Sávio; Paiva,Samuel Rezende; Silva,Priscila Vendramini; Guimarães,Simone Eliza Facioni. |
| This study investigated the association between IGF-I microsatellite marker in an F2 population (N=459) generated by mating of native boars to Brazilian commercial sows with performance, carcass cut, and meat quality traits. Association analyses were carried out using a statistical model that included genotype, sex, and group as fixed effects and sire as random effect. The IGF-I genotypes were significantly associated with different quantitative traits and these results corroborate with previous QTL analyses obtained for this chromosome region in swine. Additive and dominance effects, as well as a genotype-sex interaction, were estimated and discussed in the text. According to the results obtained, this marker is suitable for QTL search in the genotyped... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Divergent cross; Microsatellite marker; Quantitative traits; Sex × genotype interaction; Swine. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000200007 |
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| Miranda-Furtado,Cristiana Libardi; Silva,Priscila Vendramini; Guimarães,Marta Fonseca Martins; Serão,Nicola Vergara Lopes; Guimarães,José Domingos; Guimarães,Simone Eliza Facioni. |
| The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candidate gene; Litter size; Ovulation rate; Pig production; Quantitative real-time PCR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000500012 |
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| Peixoto,Jane de Oliveira; Faria,Danielle Assis de; Silva,Priscila Vendramini; Fonseca,Isabela; Lopes,Paulo Sávio; Guimarães,Simone Eliza Facioni. |
| It was investigated the association between leptin gene single nucleotide polymorphisms (SNPs) T2411C and T3266G with carcass traits in F2 pigs obtained by crossing native Brazilian Piau with commercial sows composed of Landrace, Large White and Pietrain breeds. Association analyses were performed using a statistical model that included genotype, sex, and batch as fixed effects, sire and error as random effects. The T2411C SNP was associated with skinless and fatless boston shoulder weight (BSW), backfat thickness at last rib, 6.5 cm from the midline (P2), skinless and fatless picnic shoulder weight, and sirloin weight (SLW). The T3266G mutation was associated with slaughter age, bacon weight, BSW, midline backfat thickness between last and last but one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candidate gene; Pig genomics; Pig production. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000200008 |
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