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	Provedor de dados Ciência Rural (28) BJM (3) Autor Silva,Rodrigo Otávio Silveira (31) Lobato,Francisco Carlos Faria (28) Pires,Prhiscylla Sadanã (12) Salvarani,Felipe Masiero (10) Assis,Ronnie Antunes de (7) Oliveira Junior,Carlos Augusto (7) Alves,Guilherme Guerra (4) Guedes,Roberto Maurício Carvalho (4) Facury Filho,Elias Jorge (3) Guedes,Roberto Maurício de Carvalho (3) Oliveira Júnior,Carlos Augusto de (3) Ramos,Carolina Pantuzza (3) Xavier,Rafael Gariglio Clark (3) Carvalho Filho,Maurício Baltazar de (2) Cruz Júnior,Eduardo Coulaud da Costa (2) Diniz,Amanda Nádia (2) Gabardo,Michelle de Paula (2) Gonçalves,Luciana Aramuni (2) Leal,Carlos Augusto Gomes (2) Lima,Catarina Guimarães Rocha Dourado (2) Mais... Palavra-chave Clostridium botulinum (6) Diarrhea (5) Clostridium perfringens (4) Colitis (4) Enteritis (4) Dogs (3) Nosocomial diarrhea (3) Salmonellosis (3) Zoonosis (3) Aves (2) Avian (2) Canine (2) Cattle (2) Clostridium difficile (2) Enterotoxemia (2) Epsilon (2) Equine (2) Flaccid paralysis (2) Nosocomial (2) Sheep (2) Mais... Tipo do documento Info:eu-repo/semantics/article (31) Ano 2019 (4) 2018 (4) 2017 (3) 2016 (3) 2015 (4) 2014 (3) 2013 (2) 2012 (2) 2011 (1) 2010 (1) 2009 (2) 2008 (2) Mais... País Brazil (31) Idioma Inglês (25) Português (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 31 Primeira ... 1 2 ... Última Detection of toxins A/B and isolation of Clostridium difficile and Clostridium perfringens from dogs in Minas Gerais, Brazil BJM Silva,Rodrigo Otávio Silveira; Santos,Renata Lara Resende; Pires,Prhiscylla Sadanã; Pereira,Luiz Carlos; Pereira,Silvia Trindade; Duarte,Marina Carvalho; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria. The objective of this study was to detect C. difficileA/B toxins and to isolate strains of C. perfringensand C. difficile from diarrheic and non-diarrheic dogs in Brazil. Stool samples were collected from 57 dogs, 35 of which were apparently healthy, and 22 of which were diarrheic. C. difficileA/B toxins were detected by ELISA, and C. perfringensand C. difficilewere identified by multiplex PCR. C. difficileA/B toxins were detected in 21 samples (36.8%). Of these, 16 (76.2%) were from diarrheic dogs, and five (23.8%) were from non-diarrheic dogs. Twelve C. difficile strains (21.1%) were isolated, of which ten were A+B+and two were A-B-. All non-toxigenic strains were isolated from non-diarrheic animals. The binary toxin gene cdtBwas found in one strain,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nosocomial diarrhea; Small animals; Colitis; Enteritis; Canine. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100019 Mapping of the continuous epitopes displayed on the Clostridium perfringens type D epsilon-toxin BJM Alves,Guilherme Guerra; Machado-de-Ávila,Ricardo Andrez; Chávez-Olórtegui,Carlos Delfin; Silva,Rodrigo Otávio Silveira; Lobato,Francisco Carlos Faria. Abstract The epsilon toxin, produced by Clostridium perfringens, is responsible for enterotoxemia in ruminants and is a potential bioterrorism agent. In the present study, 15 regions of the toxin were recognized by antibodies present in the serum, with different immunodominance scales, and may be antigen determinants that can be used to formulate subunit vaccines. Tipo: Info:eu-repo/semantics/article Palavras-chave: Epsilon; Toxin; Enterotoxemia; Epitope; Mapping. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300570 Antimicrobial susceptibility of Clostridium perfringens isolated from piglets with or without diarrhea in Brazil BJM Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Pires,Prhiscylla Sadanã; Cruz Júnior,Eduardo Coulaud da Costa; Albefaro,Isabella Silva; Guedes,Roberto Maurício de Carvalho; Lobato,Francisco Carlos Faria. The minimum inhibitory concentration (MIC) was determined for 13 antibiotics against Clostridium perfringens isolated from Brazilian piglets. The collection of isolates was performed in June to October 2010. All isolates were susceptible to amoxicillin and ceftiofur, whereas most were resistant to tetracycline and lincomycin. Avilamycin and narasin were more effective against isolates from non-diarrheic than from diarrheic piglets. The other antimicrobials were less active in need of high concentrations to inhibit the growth of the C. perfringens type A. These results suggest the need for further studies evaluating molecular factors related to the antimicrobial resistance of C. perfringens. Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium perfringens; Piglet; Diarrhea; Antibiotic. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300027 Salmonella Typhimurium - associated meningoencephalomyelitis in a foal Ciência Rural Oliveira,Jéssica Guerra de; Ramos,Carolina Pantuzza; Rocha,Izabela de Assis; Marcelino,Sóstenes Apolo Correia; Pierezan,Felipe; Palhares,Maristela Silveira; Maranhão,Renata de Pino Albuquerque; Silva,Rodrigo Otávio Silveira; Teixeira,Raffaella Bertoni Cavalcanti. ABSTRACT: A 10-day old foal presented with a history of acute recumbency and generalized weakness, that progressed to seizure episodes and death. Post mortem examination revealed necrotizing and purulent omphalophlebitis and fibrinopurulent meningoencephalomyelitis. Salmonella Typhimurium was isolated from the central nervous system and determined to be the cause of the meningoencephalomyelitis. Due to the lack of evidence of gastrointestinal disease, the umbilical cord was considered the most likely portal of entry of the bacteria. The isolated S. Typhimurium was resistant to ampicillin and cephalotin, and partially resistant to enrofloxacin. These drugs are commonly used in the treatment of salmonellosis. This is the first report of S. Typhimurium... Tipo: Info:eu-repo/semantics/article Palavras-chave: Horse; Salmonellosis; Omphalophlebitis; Meningoencephalitis; Diarrhea. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800451 Clostridium perfringens: a review of the disease in pigs, horses and broiler chickens Ciência Rural Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Guedes,Roberto Maurício Carvalho; Lobato,Francisco Carlos Faria. Clostridium perfringens is a gram-positive, anaerobic, fermentative, spore-forming bacillus that may be found in the environment but is more commonly found as part of the microbiota of humans and animals. The bacterium is considered a common enteric pathogen; however, the pathogenesis and predisposing factors of the disease can differ among species. Therefore, specific studies are necessary for understanding the role of this pathogen, how to diagnose it and which control measures are applicable. The aim of this paper is to review the current knowledge regarding C. perfringens infections in pigs, horses and broiler chickens Tipo: Info:eu-repo/semantics/article Palavras-chave: Diarrhea; Enteritis; Equine; Swine; Poultry. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000601027 Botulism in ruminants in Brazil Ciência Rural Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Gonçalves,Luciana Aramuni; Lobato,Francisco Carlos Faria. ABSTRACT: Botulism is an intoxication caused by the ingestion of neurotoxins secreted by Clostridium botulinum. It affects all warm-blooded animals and some fishes and is characterized by progressive flaccid symmetrical paralysis. Despite the known importance of this disease in ruminants, the Brazilian literature only includes case studies and outbreak descriptions. In addition, the epidemiology of botulism in cattle has markedly changed over the past two decades. The aim of this study is to review these characteristics and to provide recent data on the occurrence of botulism in ruminants in Brazil. Tipo: Info:eu-repo/semantics/article Palavras-chave: Flaccid paralysis; Clostridium botulinum; Cattle; Buffaloes; Goats; Sheep. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801411 Padronização de um modelo de infecção por Clostridium difficile em hamsters sírios Mesocricetus auratus Ciência Rural Silva,Rodrigo Otávio Silveira; Guedes,Roberto Maurício Carvalho; Gabardo,Michelle de Paula; Oliveira Junior,Carlos Augusto; Salvarani,Felipe Masiero; Pires,Prhiscylla Sadanã; Alves,Guilherme Guerra; Lobato,Francisco Carlos Faria. O objetivo do presente trabalho foi padronizar um modelo de infecção por Clostridium difficile (ICD) em hamsters sírios (Mesocricetus auratus). Para seleção dos isolados capazes de causar letalidade, cinco animais por grupo receberam uma dose de clindamicina (30mg kg-1) por gavagem. Após 48 horas, administraram-se 107 unidades formadoras de colônia (UFC), por animal, de quatro diferentes isolados toxigênicos de C. difficile. Selecinou-se um dos isolados capazes de causar diarreia e letalidade e administrou-se 4x102; 4x104; 4x106; 4x108UFC por animal, novamente com cinco hamsters por grupo. Em todas as diluições testadas, foi possível observar a ocorrência de diarreia e morte. A maior concentração testada (4x108UFC por animal) causou óbito de 100% dos... Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium difficile; Diarreia nosocomial; Modelo animal; Infecção hospitalar. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000801415 First confirmed case of Clostridium difficile-associated diarrhea in foals in Brazil Ciência Rural Silva,Rodrigo Otávio Silveira; Moreira,Felipe Moraghi; Rezende,Jackeline Viana; Pires,Prhiscylla Sadanã; Maranhão,Renata de Pino Albuquerque; Palhares,Maristela Silveira; Lobato,Francisco Carlos Faria. Despite of the substantial role of Clostridium difficile in causing diarrhea and colitis in foals, there have been no confirmed diagnoses of disease caused by this bacteria in Brazil. In this paper, we describe confirmed cases of colitis caused by C. difficile in two foals in Brazil. Two five-month-old foals with a five-day history of diarrhea after antibiotic treatment for a respiratory disease were treated at the Veterinary Hospital of the Universidade Federal de Minas Gerais. C. difficile A/B toxins were detected, and toxigenic strains of C. difficile were isolated from the foals' feces. The treatment was based on fluid therapy and antibiotics (metronidazole and ceftiofur), and the animals experienced a gradual recovery. The association between the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Colitis; Equine; Nosocomial diarrhea. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000300018 Isolation and genotyping of Clostridium perfringens from goats in Minas Gerais, Brazil Ciência Rural Santana,Jordana Almeida; Ferreira,Ana Carolina de Andrade; Souza,Marina de Castro Campos de; Moreira,Maria Aparecida Scatamburlo; Lima,Magna Coroa; Cruz,Diogo Soares Gonçalves; Lobato,Francisco Carlos Faria; Silva,Rodrigo Otávio Silveira. ABSTRACT: Despite the known importance of Clostridium perfringens as an enteropathogen in small ruminants, little is known about the role of its additional virulence factors or the frequency of the various C. perfringens genotypes in healthy goats; this complicates the laboratory diagnosis of the infections caused by this microorganism. In light of this, the aim of the present study was to isolate and genotype C. perfringens from stool samples from healthy goats in Brazil. Stool samples from 250 apparently healthy adult goats from 17 different herds in Minas Gerais, Brazil were collected, and isolation and genotyping of C. perfringens was performed. C. perfringens type A was isolated from 189 (75.6%) goats, whereas C. perfringens types C and D were each... Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium perfringens type D; Microbiota; Epsilon; Beta. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700452 Produção e caracterização de anticorpos monoclonais contra toxina épsilon de Clostridium perfringens Tipo D Ciência Rural Freitas,Theonys Diógenes; Heneine,Luis Guilherme Dias; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Lima,Catarina Guimarães Rocha Dourado; Pires,Prhiscylla Sadanã; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria. Clostridium perfringens tipo D é o agente etiológico da enterotoxemia em ruminantes, causada pela toxina épsilon e caracterizada por edema cardíaco, pulmonar, renal e cerebral. Anticorpos monoclonais contra toxina épsilon de C. perfringens tipo D foram produzidos a partir da fusão da linhagen de mieloma P3-X63-Ag8 653 com células do baço de camundongos Balb/c imunizados com o toxóide épsilon. Seis linhagens de híbridos secretores de anticorpos monoclonais das classes e IgM e IgG foram estabelecidas. Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium perfringens; Toxina épsilon; Anticorpos monoclonais. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100046 Microbiological quality of honey from stingless bee, jandaíra (Melipona subnitida), from the semiarid region of Brazil Ciência Rural Pinheiro,Carolina de Gouveia Mendes da Escóssia; Abrantes,Maria Rociene; Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Lobato,Francisco Carlos Faria; Silva,Jean Berg Alves da. ABSTRACT: The aim of this study was to evaluate the presence of microorganisms in honey produced by the stingless bee (SB) jandaíra (Melipona subnitida) from the semiarid region of Brazil. Thirty-five samples were analyzed and all of them were positive for mesophilic bacteria, coliforms at 45°C, fungi, and yeast. Staphylococcus spp. were identified in 85.7%, while Bacillus spp. were detected in 34.3% of honey samples. DNAs of Clostridium perfringens and C. botulinum were detected in 40% and 2.8% of the samples, respectively. Salmonella spp. and C. difficile were not detected. The present research revealed a great diversity of microorganisms in honey produced by jandaíra. Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium botulinum; Clostridium perfringens; Stingless bee. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900450 Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil Ciência Rural Coura,Fernanda Morcatti; Diniz,Amanda Nadia; Oliveira Junior,Carlos Augusto; Lage,Andrey Pereira; Lobato,Francisco Carlos Faria; Heinemann,Marcos Bryan; Silva,Rodrigo Otávio Silveira. ABSTRACT: This study identified the virulence genes, pathovars, and phylogenetic groups of Escherichia coli strains obtained from the feces of dogs with and without diarrhea. Virulence genes and phylogenetic group identification were studied using polymerase chain reaction. Thirty-seven E. coli isolates were positive for at least one virulence factor gene. Twenty-one (57.8%) of the positive isolates were isolated from diarrheal feces and sixteen (43.2%) were from the feces of non-diarrheic dogs. Enteropathogenic E. coli (EPEC) were the most frequently (62.2%) detected pathovar in dog feces and were mainly from phylogroup B1 and E. Necrotoxigenic E. coli were detected in 16.2% of the virulence-positive isolates and these contained the cytotoxic necrotizing... Tipo: Info:eu-repo/semantics/article Palavras-chave: Dogs; Escherichia coli; Pathovars; Phylogroups. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200452 Padronização da titulação da toxina épsilon de Clostridium perfringens tipo D em linhagem contínua de células como alternativa ao bioensaio animal Ciência Rural Souza Júnior,Milton Formiga; Lobato,Zélia Inês Portela; Pires,Prhiscylla Sadanã; Silva,Rodrigo Otávio Silveira; Salvarani,Felipe Masiero; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria. Enterotoxemia, também chamada de doença do rim pulposo, doença que acomete os ruminantes domésticos, é causada pela ação da toxina épsilon produzida pelo Clostridium perfringens tipo D, um anaeróbio comumente isolado do solo e das fezes de animais sadios. O método tradicional de diagnóstico baseia-se na detecção e classificação dessa exotoxina no conteúdo intestinal por meio da soroneutralização em camundongos. Com isso, o objetivo deste estudo foi padronizar um teste para detecção e titulação dessa toxina in vitro e compará-lo ao fenômeno in vivo. Para isso, uma partida de toxina épsilon de Clostridium perfringens tipo D foi titulada em camundongos e em várias linhagens contínuas de células. Após a determinação da linhagem celular mais sensível,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterotoxemia; Bioética; Soroneutralização; MDCK. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000300016 Botulism in non-ruminants in Brazil Ciência Rural Oliveira Júnior,Carlos Augusto de; Silva,Rodrigo Otávio Silveira; Olinda,Roberio Gomes; Lobato,Francisco Carlos Faria. ABSTRACT: Botulism is an intoxication caused by the ingestion of neurotoxins secreted by Clostridium botulinum and characterized by progressive flaccid symmetrical paralysis. Among non-ruminant animals, avian species and dogs are the most commonly affected by botulism, while horses and pigs are less-commonly diagnosed with the disease. Despite the importance of this disease in animals, the Brazilian literature only includes case studies and outbreak descriptions. The aim of this study was to review the main features and to provide recent data on the occurrence of botulism in non-ruminants in Brazil. Tipo: Info:eu-repo/semantics/article Palavras-chave: Flaccid paralysis; Clostridium botulinum; Avian; Dogs; Horses; Pigs. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202158 Botulismo em ruminantes causado pela ingestão de cama-de-frango Ciência Rural Lobato,Francisco Carlos Faria; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Souza,Andréa Márcia de; Lima,Catarina Guimarães Rocha Dourado; Pires,Prhiscylla Sadanã; Assis,Ronnie Antunes de; Azevedo,Edisio Oliveira de. Botulismo é uma intoxicação causada pela ingestão das toxinas produzidas pelo Clostridium botulinum, que acomete mamíferos e aves. Neste trabalho é descrito um surto de botulismo em ruminantes, ocorrido em duas propriedades localizadas no município de Patos, no Estado da Paraíba, Brasil. Em uma das propriedades, de um total de 88 bovinos, 85 (96,6%) vieram a óbito. Na segunda, morreram 145 ovinos (96,7%), 233 caprinos (57,8%) e 30 bovinos (96,8%). Os animais acometidos apresentavam paralisia progressiva, dificuldade de locomoção, sialorréia e dispnéia. A morte ocorreu entre 24 e 48 horas após o início dos sinais, por parada cardio-respiratória. Nenhuma alteração significativa foi observada no exame post-mortem. O diagnóstico de botulismo foi confirmado... Tipo: Info:eu-repo/semantics/article Palavras-chave: Botulismo; Clostridium botulinum; Cama-de-frango; Ruminantes. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000400046 Isolation and genotyping of Clostridium perfringens and Clostridium difficile in Capuchin Monkeys (Sapajus spp.) Ciência Rural Silva,Rodrigo Otávio Silveira; Ferreira,Débora Rochelly Alves; Laroquec,Plautino de Oliveira; Xavier,Rafael Gariglio Clark; Lobato,Francisco Carlos Faria; Mota,Rinaldo Aparecido. ABSTRACT: The importance of Clostridium perfringens and C. difficile for most wild animal species remains unclear. This study aimed to isolate and genotype C. perfringens and C. difficile in stool samples from free-living and captive capuchin monkeys (Sapajus flavius and Sapajus libidinosus) in Brazil. Ten free-living S. flavius and 14 captive S. libidinosus were sampled for this study. To isolate C. difficile, stool samples were inoculated on plates containing cycloserine-cefoxitin fructose agar supplemented with horse blood and sodium taurocholate. Two different protocols for C. perfringens isolation were tested: direct plating onto selective agar and enrichment in brain heart infusion (BHI) broth followed by plating onto selective agar. C. difficile was... Tipo: Info:eu-repo/semantics/article Palavras-chave: New World Primates (NWP); Enterocolitis; Sapajus libidinosus; Sapajus flavius. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200452 A retrospective study on the diagnosis of clostridial myonecrosis in ruminants in Brazil Ciência Rural Pires,Prhiscylla Sadanã; Ecco,Roselene; Silva,Rodrigo Otávio Silveira; Araújo,Marina Rios de; Salvarani,Felipe Masiero; Heneine,Luíz Guilherme Dias; Oliveira Júnior,Carlos Augusto de; Lobato,Francisco Carlos Faria. ABSTRACT: A standardized immunochemistry method for the diagnosis of clostridial myonecrosis was applied to 38 formalized tissue samples from ruminants with clinical and post mortem history suggestive of blackleg or gas gangrene. The diagnosis of clostridial myonecrosis was confirmed in 37 out of 38 (97.4%) samples tested. Clostridium chauvoei and Clostridium perfringens type A were the most common agents found alone, being detected in ten (26.3%) and six (15.8%) samples, respectively. The other cases showed an association of two or three clostridia, with C. perfringens type A detected in 11 (29%) cases. Based on the findings of the present study, polyvalent vaccines against clostridial infections of animals incorporating C. perfringens would be more... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gas gangrene; Blackleg; Malignant edema; Cattle; Sheep; Immunohistochemistry. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100452 Clostridium difficile infection: main features and occurrence in domestic species in Brazil Ciência Rural Silva,Rodrigo Otávio Silveira; Guedes,Roberto Maurício de Carvalho; Lobato,Francisco Carlos Faria. Clostridium difficile is an emerging enteropathogen of humans and domestic animals. The bacterium was recently confirmed to be present in foals and dogs in Brazil, with some recent studies suggesting that C. difficile is one of the most important causes of piglet diarrhea in the country. Moreover, some reports also suggest the transmission of this microorganism between animals and humans, raising the possibility that C. difficile is a zoonotic disease. Therefore, the aim of the present review is to describe the main features of C. difficile infection in domestic animals and outline the occurrence of the disease in horses, dogs and pigs in Brazil. Tipo: Info:eu-repo/semantics/article Palavras-chave: Enteritis; Zoonosis; Clostridiosis; Nosocomial; Piglets; Foals; Dogs. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100013 Antimicrobial susceptibility and molecular characterization of Salmonella serovar Ndolo isolated from outbreaks in cattle and horses Ciência Rural Ramos,Carolina Pantuzza; Xavier,Rafael Gariglio Clark; Leal,Carlos Augusto Gomes; Facury Filho,Elias Jorge; Carvalho,Antonio Ultimo de; Viegas,Flávia Mello; Pires,Isadora Honorato; Lopes,Emily Oliveira; Lobato,Francisco Carlos Faria; Silva,Rodrigo Otávio Silveira. ABSTRACT: The present study aimed to describe and characterize, for the first time, two outbreaks of salmonellosis caused by Salmonella Ndolo in foals and calves in Brazil and compare the isolated strains with S. Ndolo previously identified in asymptomatic reptiles. The affected calves and foals presented fever, lethargy, and profuse diarrhea. Isolated strains were subjected to antimicrobial susceptibility testing, characterized according to virulence genes, and fingerprinted by ERIC-PCR. Salmonella Ndolo was identified in fecal samples from two foals and four calves. One isolate from a calf was resistant to amoxicillin/clavulanic acid, trimethoprim/sulfamethoxazole, and florfenicol. Strains from two other calves were resistant to oxytetracycline. All... Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonellosis; Zoonoses; Antimicrobial resistance; ERIC-PCR; Salmonella Ndolo.. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200452 Synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasis Ciência Rural Rennó,Mário César; Vasconcellos,Amanda Otoni; Santos,Rogerio Rodrigues; Silva,Rodrigo Otávio Silveira; Carneiro,Rubens Antônio; Paes,Paulo Ricardo de Oliveira. ABSTRACT: Leishmaniasis represents a complex of chronic diseases with a broad geographic distribution and a high significance in public health worldwide. The varied clinical signs in conjunction with the low sensitivity and specificity of canine visceral leishmaniasis (CVL) detection methods make diagnosis of the disease complex. Among the several available laboratory tests, studies have suggested that the detection of parasites in synovial fluid (SF) is a good auxiliary tool in the diagnosis of CVL. However, no study has evaluated the influence of the clinical stage of CVL in the detection of Leishmania sp. in SF. This study aimed to evaluate the detection of Leishmania sp. amastigotes in the SF of dogs at different stages of the disease. The negative... Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmania infantum; Zoonosis. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500901 Registros recuperados: 31 Primeira ... 1 2 ... Última

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