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| Serravalle,Karina; Santos,Norma; Sardi,Silvia Inês; Silva,Sarah Peregrino Santos; Ribeiro Junior,Hugo da Costa; Mattos,Ângela Peixoto de; Campos,Gúbio Soares. |
| Rotavirus is a major cause of infectious diarrhea in infants and young children. The objective of this study was to characterize the genotypes of Human Rotavirus found in children hospitalized with acute diarrhea in the Pediatric Hospital Prof. Hosannah de Oliveira of the UFBA in Salvador, Bahia, Brazil, during the years of 1999, 2000 and 2002. Fecal samples were analyzed (n=358) by methods EIARA and SDS-PAGE for detection of Rotavirus. Positive samples of one or two of these methods (n=168) were submitted to RT-PCR and Multiplex-Nested PCR to determine genotypes G and P. A hundred sixty-eight (46.9%) samples were positive and 190 (53.1%) negative. Only 17 (4.7%) samples had divergent results. The distribution of genotypes G during the first year, showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; PCR; Genotype; Children. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100010 |
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