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Secondary structure of nrDNA Internal Transcribed Spacers as a useful tool to align highly divergent species in phylogenetic studies Genet. Mol. Biol.
Giudicelli,Giovanna C.; Mäder,Geraldo; Silva-Arias,Gustavo A.; Zamberlan,Priscilla M.; Bonatto,Sandro L.; Freitas,Loreta B..
Abstract Recently, it has been suggested that internal transcribed spacer (ITS) sequences are under selective constraints to preserve their secondary structure. Here, we investigate the patterns of the ITS nucleotide and secondary structure conservation across the Passiflora L. genus to evaluate the potential use of secondary structure data as a helpful tool for the alignment in taxonomically complex genera. Considering the frequent use of ITS, this study also presents a perspective on future analyses in other plant groups. The ITS1 and ITS2 sequences presented significant differences for mean values of the lowest energy state (LES) and for number of hairpins in different Passiflora subgenera. Statistical analyses for the subgenera separately support...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passiflora; Lowest energy state; Hairpins; ITS1; ITS2.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200191
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Phylogenetic niche conservatism and plant diversification in South American subtropical grasslands along multiple climatic dimensions Genet. Mol. Biol.
Barros,Michel J.F.; Silva-Arias,Gustavo A.; Segatto,Ana Lúcia Anversa; Reck-Kortmann,Maikel; Fregonezi,Jeferson N.; Diniz-Filho,José Alexandre F.; Freitas,Loreta B..
Abstract Phylogenetic niche conservatism can be investigated at multiple scales on an explicit geographical context. Haplotype-based comparative analyses of lineages occupying the same region, and thus subjected to similar environmental factors, allow decoupling shared evolutionary and ecological patterns, as well as multiple dimensions of adaptive diversification. Here we aimed to assess the role of environmental drivers on diversification of subtropical grassland, based on haplotypic diversity of two plant genera. We sampled two closely related and co-distributed grassland plant genera, Petunia and Calibrachoa, across their entire distribution area. Eigenvectors extracted from pairwise distances based on chloroplast DNA haplotypes were used to fit...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Altitude; Climatic changes; Molecular diversification; Phylogenetic niche conservatism.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400602
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