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Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil BJMBR
Sousa-Junior,F.C. de; Silva-Carvalho,M.C.; Fernandes,M.J.B.C.; Vieira,M.F.P.; Pellegrino,F.L.P.C.; Figueiredo,A.M.S.; Melo,M.C.N. de; Milan,E.P..
Methicillin-resistant Staphylococcus aureus (MRSA) is a major agent of hospital infections worldwide. In Brazil, a multiresistant MRSA lineage (ST239-SCCmecIIIA), the so-called Brazilian epidemic clone (BEC), has predominated in all regions. However, an increase in nosocomial infections caused by non-multiresistant MRSA clones has recently been observed. In the present study, 45 clinical isolates of MRSA obtained from a university hospital located in Natal city, Brazil, were identified by standard laboratory methods and molecularly characterized using staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis. Antimicrobial susceptibility testing was carried out using CLSI methods. The MRSA isolates studied displayed a...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Methicillin-resistant Staphylococcus aureus; MRSA; Antimicrobial resistance; Brazilian epidemic clone; Pediatric clone; SCCmecIV.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000002
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Emergence of clonal complex 5 (CC5) methicillin-resistant Staphylococcus aureus (MRSA) isolates susceptible to trimethoprim-sulfamethoxazole in a Brazilian hospital BJMBR
Teixeira,M.M.; Araújo,M.C.; Silva-Carvalho,M.C.; Beltrame,C.O.; Oliveira,C.C.H.B.; Figueiredo,A.M.S.; Oliveira,A.G..
In this study, genotyping techniques including staphylococcal chromosomal cassette mec (SCCmec) typing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and restriction-modification tests were used to compare the molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered at two times within a 10-year interval (1998 and 2008) from a tertiary Brazilian hospital. In addition, the antimicrobial susceptibility profiles were analyzed. All 48 MRSA isolates from 1998 and 85.7% from 2008 (48/56 isolates) displayed multidrug-resistance phenotypes and SCCmec III. All but one of the 13 representative SCCmec III isolates belonged to CC8 and had PFGE patterns similar to that of the BMB9393 strain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Antimicrobial susceptibility; CC5; Cordobes clone; Trimethoprim-sulfamethoxazole.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700011
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The first report in Brazil of severe infection caused by community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) BJMBR
Rozenbaum,R.; Sampaio,M.G.; Batista,G.S.; Garibaldi,A.M.; Terra,G.M.F.; Souza,M.J.; Vieira,E.N.; Silva-Carvalho,M.C.; Teixeira,L.A.; Figueiredo,A.M.S..
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emergent pathogen in Brazil. However, there are no data on the prevalence of CA-MRSA. We report here the first well-characterized case of severe life-threatening CA-MRSA infection in a child living in Rio de Janeiro city. The patient had many complications including hematogenous osteomyelitis and involvement of multiple sites requiring drainage of soft-tissue abscess, and pleural and pericardial empyema. The MRSA isolates recovered were genotyped using PFGE, SCCmec typing and multilocus sequence typing. Disk diffusion tests were performed following Clinical and Laboratory Standards Institute recommendations. In addition, the presence of Panton-Valentine leukocidin (PVL) was...
Tipo: Info:eu-repo/semantics/other Palavras-chave: CA-MRSA; MRSA; PVL; S. aureus; Antimicrobial resistance.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800011
Registros recuperados: 3
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