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| Simões-Araújo,Jean Luiz; Leite,Jakson; Marie Rouws,Luc Felicianus; Passos,Samuel Ribeiro; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa; Zilli,Jerri Édson. |
| Abstract The strain BR 3262 was isolated from nodule of cowpea (Vigna unguiculata L. Walp) growing in soil of the Atlantic Forest area in Brazil and it is reported as an efficient nitrogen fixing bacterium associated to cowpea. Firstly, this strain was assigned as Bradyrhizobium elkanii, however, recently a more detailed genetic and molecular characterization has indicated it could be a Bradyrhizobium pachyrhizi species. We report here the draft genome sequence of B. pachyrhizi strain BR 3262, an elite bacterium used as inoculant for cowpea. The whole genome with 116 scaffolds, 8,965,178 bp and 63.8% of C+G content for BR 3262 was obtained using Illumina MiSeq sequencing technology. Annotation was added by the RAST prokaryotic genome annotation service and... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Atlantic forest area; Biological nitrogen fixation; Next generation sequencing; Nodulation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400783 |
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| Silva,Paula Renata Alves da; Simões-Araújo,Jean Luiz; Vidal,Márcia Soares; Cruz,Leonardo Magalhães; Souza,Emanuel Maltempi de; Baldani,José Ivo. |
| Abstract Paraburkholderia tropica (syn Burkholderia tropica) are nitrogen-fixing bacteria commonly found in sugarcane. The Paraburkholderia tropica strain Ppe8 is part of the sugarcane inoculant consortium that has a beneficial effect on yield. Here, we report a draft genome sequence of this strain elucidating the mechanisms involved in its interaction mainly with Poaceae. A genome size of approximately 8.75 Mb containing 7844 protein coding genes distributed in 526 subsystems was de novo assembled with ABySS and annotated by RAST. Genes related to the nitrogen fixation process, the secretion systems (I, II, III, IV, and VI), and related to a variety of metabolic traits, such as metabolism of carbohydrates, amino acids, vitamins, and proteins, were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diazotrophic; Next generation sequencing; Nitrogen-fixing; Secretion system. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200210 |
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| Simões-Araújo,Jean Luiz; Rumjanek,Norma Gouvêa; Margis-Pinheiro,Márcia. |
| Plants respond to temperature stress by synthesizing a set of heat shock proteins (HSPs), which may be responsible for the acquisition of thermotolerance. In this study, the induction of small HSPs (sHSPs) in eight common bean varieties was evaluated by Northern blot analysis using the W HSP 16.9 cDNA as heterologous probe. Cowpea was used, as a positive control since this plant, as opposed to common bean, is known to grow well under high temperature regimes such as that found in the Brazilian semi-arid region. After the growth period, the plants were submitted to two h of heat shock at 40 ºC. All varieties tested were able to induce sHSP mRNAs that hybridized with W HSP 16.9 probe. However, significant kinetic differences were found when comparing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Common bean; Heat shock proteins; HSPs; Thermotolerance; Temperature stress. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000100005 |
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| Silva,Helder Anderson Pinto da; Galisa,Péricles de Souza; Oliveira,Roselaine Sanches da Silva; Vidal,Marcia Soares; Simões-Araújo,Jean Luiz. |
| O objetivo deste trabalho foi avaliar o efeito isolado ou simultâneo dos estresses hídrico e térmico na expressão gênica em nódulos de feijão-caupi. A bactéria Bradyrhizobium japonicum (estirpe BR 3267) foi inoculada em sementes de feijão-caupi da cultivar IPA 206 e, 35 dias após a germinação, as plantas foram submetidas a diferentes regimes de disponibilidade hídrica e a estresse térmico, em casa de vegetação. Para a identificação dos genes diferencialmente expressos, foi utilizada a técnica de cDNA-AFLP, tendo-se isolado 67 fragmentos derivados de transcritos (FDTs) diferencialmente expressos. Após o sequenciamento dos FDTs e das análises de similaridade, com uso do programa Blastx, foram identificados 14 genes diferencialmente expressos envolvidos em... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vigna unguiculata; CDNA-AFLP; Fixação biológica de nitrogênio; Genômica funcional. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000600010 |
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