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| Michelon,André; Michelon,Marcelo; Simionatto,Simone; Lagranha,Valeska Lizzi; Conceição,Fabrício Rochedo; Vaz,Eliana Knackfus; Cerqueira,Gustavo Maia; Dellagostin,Odir Antonio. |
| The neonatal diarrhea in swine caused by enterotoxigenic Escherichia coli (ETEC) is responsible for high mortality and low growth rate in pigs and it is mainly dependent on the capacity of E. coli to attach to the surface of the small intestine, a property mediated by fimbria. In this study the faeC gene, which codes for the minor fimbrial subunit of E. coli K88ab, was cloned in the eukaryotic expression vector pcDNA3, associated or not to the Kozak sequence. Plasmid DNA of the two versions of the vaccine candidate was inoculated in mice by the intramuscular route, in two doses, at 0 and 21 days. The animals that received the DNA vaccine containing faeC associated to the Kozak sequence presented seroconversion significantly higher (P<0.05) than the one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Swine colibacilosis; DNA vaccine; FaeC; Kozak sequence. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500029 |
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| Nassi,Fernanda; Seixas,Fabiana Kömmling; Jouglard,Sandra Denize Dorneles; Simionatto,Simone; Silva,Everton Fagonde; Seyffert,Núbia; Brod,Claudiomar Soares; Dellagostin,Odir Antonio. |
| Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods. The aim of this work was to evaluate and optimize a Nested-PCR method for diagnosis of leptospirosis. Primers were designed to amplify a 264 bp region within the LipL32 gene. The sensitivity and specificity of the assay was evaluated using seven saprophytic serovars and 35 pathogenic serovars. This technique showed to be very specific for pathogenic serovars, however it lacked sensitivity. In order to enhance the sensitivity,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500031 |
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