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| Simoni,Isabela Cristina; Fernandes,Maria Judite Bittencourt; Custódio,Renata Marconi; Madeira,Alda Maria Backx Noronha; Arns,Clarice Weis. |
| The susceptibility of the five cell lines - IB-RS-2, RK-13, Vero, BHK-21, CER - to reovirus S1133 and infectious bursal disease virus (IBDV vaccine GBV-8 strain) was studied to better define satisfactory and sensitive cell culture systems. Cultures were compared for presence of CPE, virus titers and detection of viral RNA. CPE and viral RNA were detected in CER and BHK-21 cells after reovirus inoculation and in RK-13 cell line after IBDV inoculation and with high virus titers. Virus replication by production of low virus titers occurred in IB-RS-2 and Vero cells with reovirus and in BHK-21 cell line with IBDV. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reovirus; Infectious bursal disease virus; Cell lines; Susceptibility. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400015 |
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| Fernandes,Maria Judite Bittencourt; Simoni,Isabela Cristina; Harakava,Ricardo; Rivas,Eliana Borges; Arns,Clarice Weis. |
| Infectious bursal disease virus (IBDV) is classified according to the antigenicity and virulence into classical virulent (cv), very virulent (vv), and antigenic variant strains. The molecular basis for the IBDV antigenic variation is well established and is associated to the capsid protein, VP2 (gene VP2 of segment A), whereas both VP2 and the RNA-dependent RNA polymerase, VP1 (gene VP1 of segment B), have been correlated with the virulence. In this study, seventeen Brazilian IBDV samples previously characterized by the VP2 gene as cv (three) and vv (fourteen) strains were genetically and molecularly analyzed for their VP1 gene. All of the strains kept with the same cv or vv classification except one sample, Br/03/DR. This sample was classified as vv by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian infectious bursal disease virus; Very virulent strain; VP1; Sequencing; Phylogenetic analysis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300024 |
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| Pak,Adriana; Gonçalez,Edlayne; Felicio,Joana D'arc; Pinto,Marina Mori; Rossi,Maria Helena; Simoni,Isabela Cristina; Lopes,Márcia Nasser. |
| Polymnia sonchifolia, commonly known as ";yacon";, was originally cultivated at Andes moutains in South America. Recently, the specie attracted worldwide attention because of its wide range of uses, for example in the control of diabetes melitus, besides the antifungal and pesticidal compounds were found in the leaves. This study describes the identification of two flavonoids: 3', 5, 7 trihydroxy-3, 4'-dimethoxyflavone (compound 1) and 3', 4', 5- trihydroxy-7-methoxy flavanone (compound 2) and two sesquiterpenes lactones: enhydrin (compound 3) and a mixture of enhydrin and uvedalin (compound 4) isolated from Polymnia sonchifolia leaves and their effects on the aflatoxin production by Aspergillus flavus. The identification of the compounds were achieved by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymnia sonchifolia; Flavonoids; Melampolides; Aflatoxin; Aspergillus flavus. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200018 |
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