Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJM (2) Autor Gomes,Newton C.M. (2) Smalla,Kornelia (2) Campos,Tácio M.P. de (1) Chaloub,Ricardo Moreira (1) Costa,Rodrigo (1) Dealtry,Simone (1) Ghizelini,Angela Michelato (1) Mendonça-Hagler,Leda C.S. (1) Milling,Annett (1) Reinert,Fernanda (1) Mais... Palavra-chave IncP-9 plasmids (1) Mangrove (1) Microbial communities (1) Ndo genes (1) Nucleic acids (1) Phytoremediation (1) RNA extraction (1) Rhizosphere (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2004 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Petroleum contamination and bioaugmentation in bacterial rhizosphere communities from Avicennia schaueriana BJM Dealtry,Simone; Ghizelini,Angela Michelato; Mendonça-Hagler,Leda C.S.; Chaloub,Ricardo Moreira; Reinert,Fernanda; Campos,Tácio M.P. de; Gomes,Newton C.M.; Smalla,Kornelia. ABSTRACT Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana against artificial petroleum contamination in a phytoremediation greenhouse experiment. Interestingly, despite plant protection due to the inoculation, denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene fragments amplified from the total community DNA indicated that the different treatments did not significantly affect the bacterial community composition. However, while... Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytoremediation; Mangrove; IncP-9 plasmids; Ndo genes. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400757 An optmized protocol for simultaneous extraction of DNA and RNA from soils BJM Costa,Rodrigo; Gomes,Newton C.M.; Milling,Annett; Smalla,Kornelia. In this work we report an optimized protocol for simultaneous extraction of DNA and RNA from soil matrices. Treatment of soil matrices with ethanol followed by bead-beating worked as a successful strategy to lyse the cells without considerable degradation of nucleic acids, resulting in DNA and RNA of good yield and integrity. The reverse transcribed RNA could be amplified with primers targeting a glutamine synthetase (glnA) gene fragment. From both DNA and cDNA, 16S rDNA fragments were amplified and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE). The method was applied to soil and rhizosphere (strawberry and oilseed rape) samples. Two other protocols for the extraction of nucleic acids from soil were applied to the same set of samples in order... Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA extraction; Nucleic acids; Rhizosphere; Microbial communities. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200011 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco