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Cecchini,A.L.; Soares,A.M.; Giglio,J.R.; Amara,S.; Arantes,E.C.. |
This paper describes a brief study on the crotoxin mechanism of action, regarding the transport of GABA and L-glutamate in rats cortico-cerebral synaptosomes and in heterologous systems, such as COS-7 cells expressing gabaergic transporters, and C6 glioma cells and Xenopus oocytes expressing glutamatergic transporters. Crotoxin concentrations over 1 µM caused an inhibitory effect of ³H-L-glutamate and ³H-GABA, and reversibly inhibited L-glutamate uptake by C6 glioma cells. When COS-7 cells were assayed, no inhibition of the ³H-GABA transport could be evidenced. Crotoxin kept its inhibitory effect on neurotransmitters uptake even when Ca2+ ions were removed from the medium, therefore, independently of its PLA2 activity. In addition, high concentrations (2... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotoxin; L-glutamate; GABA; Crotalus durissus terrificus. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300006 |
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Sampaio,I.C.; Medeiros,P.H.Q.S.; Rodrigues,F.A.P.; Cavalcante,P.A.; Ribeiro,S.A.; Oliveira,J.S.; Prata,M.M.G.; Costa,D.V.S.; Fonseca,S.G.C.; Guedes,M.M.; Soares,A.M.; Brito,G.A.C.; Havt,A.; Moore,S.R.; Lima,A.A.M.. |
Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether a multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological and functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control mice were fed a standard diet and II) undernourished mice were fed the RBD. After 7 days, mice were killed and the ileum collected for evaluation of electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) and protein expression (western blotting) of intestinal transporters and tight junctions. Body weight gain was significantly decreased in the undernourished group, which also showed decreased crypt depth but no... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Undernutrition; Ion transport; Intestinal absorption. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000607 |
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