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| Registros recuperados: 16 | |
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| Soccol,Carlos Ricardo; Pollom,Teresinha E. V.; Fendrich,Ricardo Cancio; Prochmann,Fernando Alberto; Mohan,Radijiskumar; Blaskowski,Marita Maciel Moreira; Melo,André Luiz de Almeida; Carvalho,Cláudio Jose Barros de; Soccol,Vanete Thomaz. |
| Aedes aegypti is the vector of Dengue disease, responsible for 20,000 deaths/year worldwide. Bacillus thuringiensis var israelensis - Bti releases selective and effective toxins (crystal proteins) against A. aegypti larvae. We present a low cost bioprocess for toxin production, accomplished by a selected Brazilian strain Bti (BR-LPB01) and employment of low cost substrates. Soybean meal and sugarcane molasses lead to high toxic effectiveness after 2L bioreactor fermentation (LD50=26ng/mL), near to the reference strain IPS82 (LD50=17.3 ng/mL). The pH ranged between 5.8 and 7.0 during the exponential growth period and between 7.0 and 8.4 during the stationary phase, with low activity. Thus, control of foam and pH 7.0 were started and proved to be crucial for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biopesticide; BTI; Dengue Fever Disease; Soybean meal; Sugarcane molasses. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700017 |
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| Salmon,Denise Naomi; Piva,Luíza Cesca; Binati,Renato Leal; Vandenberghe,Luciana Porto de Souza; Soccol,Vanete Thomaz; Soccol,Carlos Ricardo; Spier,Michele Rigon. |
| A new formulated product containing high yield of phytase from Schizophyllum sp., an important mushroom used for medicinal studies, was developed for application in feed industries and for future use in food processing. The enzyme presented a high activity yield 55.5 U/mL and 6240 U/gds in liquid and solid formulated product, respectively. It showed a good shelf-life in concentrated product, retaining 67.8% of its activity after 60 days of storage at room temperature and 90% of the activity was maintained in the liquid formulation after the same period. Powder bioformulated product maintained 77% of its activity after two months of storage, without the addition of chemical additives, which was named as a new bioformulated product containing high quantities... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phytase; Shizophyllum sp; Downstream steps; Stability; Formulation. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600001 |
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| Arakaki,Andréa Haruko; Souza Vandenberghe,Luciana Porto de; Soccol,Vanete Thomaz; Masaki,Ryu; Rosa Filho,Ernani Francisco da; Gregório,Alexsandro; Soccol,Carlos Ricardo. |
| The objective of this work was to study the production of biomass with copper bioaccumulation in submerged fermentation using sugarcane molasses. Candida pelliculosa BARU 05 isolated from Baru (Dipteryx alata) was selected for its good capacity to accumulate the copper. Fermentation was carried out using the medium composed by sugarcane molasses at 5 °Brix enriched with (g/L) CuSO4.5H2O 0.1; yeast extract, 10.0; (NH4)2SO4, 5.0 ; KH2PO4, 5.0 MgSO4, 0.5, inoculum 10 % of total volume (100 ml), pH 6.0, and incubation at 30 °C, 120 rpm for 120 h. After three steps of optimization an uptake of 95.04% and 13.397 g/L biomass were obtained. The kinetics of copper bioaccumulation and biomass production was followed in a 10- liter bioreactor in a batch and fed-batch... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioaccumulation; Baru; Yeast; Copper; Sugarcane molasses. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500021 |
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| Walter,Alfredo; Carvalho,Júlio Cesar de; Soccol,Vanete Thomaz; Faria,Ana Bárbara Bisinella de; Ghiggi,Vanessa; Soccol,Carlos Ricardo. |
| The aim of this work was to investigate the production of phycocyanin by Spirulina platensis under different spectra of light. The dependent variables evaluated were the amount of phycocyanin obtained and its purity, demonstrating that there might be a restructuring of phycobilisomes, especially when the culture was subjected to red light, which increased the purity level up to 33% with a reduction of 16% in phycocyanin content, but with higher photosynthetic efficiency compared to natural light. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spirulina; Phycocyanin; Phycobilisome; Pigment; Spectrum; Chromatic acclimation. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400005 |
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| Minozzo,João Carlos; Gusso,Rubens L. Ferreira; Castro,Edilene A. de; Lago,Oscar; Soccol,Vanete Thomaz. |
| Studies were carried out on the recovery rate and cysticerci location in bovines experimentally infected with Taenia saginata eggs. Three calves of 6.5 months and one with 19 months of age were infected orally with 2 x 10(4) eggs of Taenia saginata. A fifth calf served as control. After 90 days of infection, the animals were slaughtered and organs and skeletal muscles were inspected using a slicing technique every 5 mm. From the four infected calves, 702 cysticerci were recovered, of which 570 (81.2%) were alive and 132 (18.8%) were degenerated. The recovery rate ranged from 0.01 to 1.43% with an average of 0.88%. The cysticerci presented the following anatomical distribution: hioideos muscles 02 (0.28%), kidneys 03 (0.43%), tongue 07 (1.00%), liver 12... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Taenia saginata; Cysticercus bovis; Cysticerci location. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600008 |
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| Bordignon Junior,Sidnei Emílio; Miyaoka,Mitiyo Fukuda; Spier,Michele Rigon; Rubel,Rosália; Soccol,Vanete Thomaz; Soccol,Carlos Ricardo. |
| The aim of this study was to investigate the potential of lactic acid bacteria, bacilli and yeasts isolates to produce antimicrobial substances, especially against Gram-negative bacteria isolated from the animal faeces, searching for a new alternative to control the enteritis diseases in animal health. Evaluations were performed by microdilution in broth using crude extract obtained from the cultivation of 272 strains against Escherichia coli, S. Typhimurium, S. Enteritidis and Pseudomonas aeruginosa. Thirty-five lactic acid bacteria presented some antimicrobial action. One Lactobacillus isolate, named FLPB-1, was selected to studies the kinetics of the production. An important increase in the production was obtained when the producer strain had... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial; Enteritis; Lactic acid bacteria; Bacteriocin. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400011 |
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| Ribeiro,Maria Carolina de Oliveira; Vandenberghe,Luciana Porto de Souza; Spier,Michele Rigon; Paludo,Katia Sabrina; Soccol,Carlos Ricardo; Soccol,Vanete Thomaz. |
| The aim of this study was to evaluate the probiotic properties of Pediococcus acidilactici B14 and to study its resistance in the gastrointestinal system when combined with Lactobacillus acidophilus ATCC 4356 and used in a potentially symbiotic aerated soy based dessert. P. acidilactici B14 showed some important probiotic characteristics such as survival rate of 45.9% at pH 2.5; 72.4% in 0.3% bile salts and 95.8% after gastrointestinal transit at pH 4.0. Tolerance against the antibiotics cephalexin, neomycin, vancomycin, cefotaxime and penicillin G was also observed. The strain inhibited antagonism against the following cultures: Escherichia coli ATCC 25922, Bacillus cereus ATCC 33018, Staphylococcus aureus ATCC 6538P and Salmonella sp. The mixed culture... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pediococcus acidilactici; Lactobacillus acidophilus; Gastrointestinal system; Probiotics; Soy. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500755 |
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| Zoz,Liliana; Carvalho,Julio Cesar; Soccol,Vanete Thomaz; Casagrande,Thais Costa; Cardoso,Ligia. |
| Torularhodin and torulene are two widespread microbial carotenoids with relatively few studies, as compared to other nutraceutical carotenoids such as β-carotene, lycopene and astaxanthin. Several genera of microorganisms produce it in high concentration (up to 0.1% of the cell dry weight), probably as a protection against photooxidation and free radicals. These pigments, which differ by a terminal carboxylic group, have provitamin-A activity and, being red, have potential use as food and cosmetic color additives. Several factors affect the biosynthesis of these substances, including: the composition of culture media, light irradiation, which may enhance the carotenoid production up to 25% of the non-irradiated cultures, and temperature, which changes the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carotenoid; Antioxidant; Microorganism; Biopigment. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200278 |
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| Kruger,Ernesto Renato; Penha,Tania Regina; Stoffelo,Daura Regina Eira; Roehe,Paulo Michel; Ribeiro,Magda Costa; Soccol,Vanete Thomaz. |
| Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinaesub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoHV-4 virus; PCR; RFLP; Fingerprint. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100279 |
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| Netto,Cristiane; Soccol,Vanete Thomaz; Sepulveda,Lya Madureira; Timenetsky,Jorge. |
| Mycoplasma spp, belongs to the class Mollicutes and is capable to produce alterations in cellular cultures causing damages to the biotechnological industry. Bioproducts generally require two essential inputs, bovine serum and cells. The study herein aims to evaluate the mycoplasma concentrations that affect the growing of BHK21 and Vero cells. The species used were: Mycoplasma orale, M. salivarium, M. arginini and M. hyorhinis, cultivated in a SP4 media. Two contamination tests were performed with BHK21 and Vero cells and one of them applied different concentrations of mycoplasma. In the first one, mycoplasma was applied at the day zero and, in the second one, the contamination was performed after the monolayer establishment. The both cellular cultures... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Growth reduction; PCR; Biotechnological products. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400048 |
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| Pinto,Simone Benghi; Soccol,Vanete Thomaz; Vendruscolo,Eliane; Rochadelli,Roberto; Ribeiro,Paulo Bretanha; Freitag,Alaércio; Henemann,Carlos; Uemura,Márcio. |
| O presente trabalho foi desenvolvido na UFPR - Campus Palotina, no Paraná, e em três propriedades rurais do mesmo município, durante o período de novembro de 1997 a maio de 1999. O experimento foi delineado com os objetivos de avaliar: a) a flutuação de larvas de Dermatobia hominis (Linnaeus Jr., 1781) em bovinos da raça Holandesa Preto e Branco criados no município de Palotina, e a distribuição desses ectoparasitos na superfície corporal de bovinos; e b) a sazonalidade dos principais dípteros vetores de ovos de D. hominis. Para a captura dos insetos vetores, foram utilizados dois tipos de armadilhas: MAGOON e orientada pelo vento (wind oriented trap = WOT). Um total de 346 larvas foram expulsas por meio de compressão manual, sendo que a intensidade de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dermatobia hominis; Larvas; Bovinos; Sazonalidade; Vetores. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000500013 |
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| Registros recuperados: 16 | |
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