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| Nandkumar Kamat; Sonashia Velho-Pereira. |
| New molecule discovery from natural sources, such as that of actinobacteria, has proved to be an interesting area in antibiotic research, as most of these antibiotics are difficult to synthesize. Out of 30 actinobacterial cultures screened for antimicrobial activity, 28 cultures were found to produce active products against various pathogenic microorganisms such as Gram-negative, Gram-positive bacteria and yeast, using a ‘modified cross streak method.' The modified method helped in easy quantification of results and also in ruling out probable mutual antibiosis. 53%, 13% and 10% of tested actinobacterial strains belonging to Streptomyces, Micromonospora and Actinomadura genera, respectively, showed the ability of producing antimicrobial... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Cancer; Chemistry; Immunology; Microbiology; Pharmacology; Bioinformatics. |
| Ano: 2012 |
URL: http://precedings.nature.com/documents/6765/version/1 |
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| Sonashia Velho-Pereira; Nandkumar M. Kamat. |
| Soils are known to be ultimate and complex reservoirs of microbial diversity. The complex dimensions of bacterial and fungal diversity in tropical soils and microbial community dynamics are underexplored. Isolation techniques aimed at Actinomycetes generally employ highly selective media, powerful antibiotics and antifungal compounds to suppress undesirable bacteria and fungi. However some soil fungi may show their resistance towards these antifungal compounds. During our work to explore soil actinomycetes diversity, slides coated with Arginine Vitamin agar (AVA) incorporating a cocktail of antibiotics and antifungal compounds such as Nystatin, Cycloheximide, Terbinafin, Griseofulvin, and Fluconazole were exposed to soil environment and were retrieved at... |
| Tipo: Poster |
Palavras-chave: Microbiology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4208/version/1 |
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| Sonashia Velho-Pereira; Nandkumar M. Kamat. |
| High frequency isolation of actinomycetes poses a challenge for the taxonomists hence simple and rapid identification methods are required. Our work to catalogue biodiversity of actinomycetes of Goa yielded several distinct morphotypes. After their tentative identification, the feasibility to distinguish these using digital image analyses (DIA) was explored. Digital images of wild colony morphotypes were processed using public domain SCION image analysis software. DIA revealed some intricate digital characters. A combination of these with standard morphological and microscopic characters could be potentially useful for preparing a digital identification key of the actinomycetes strains with potential application in rapid taxonomic identification. |
| Tipo: Manuscript |
Palavras-chave: Microbiology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4209/version/1 |
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