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| Song, Yun-peng; Suquet, Marc; Queau, Isabelle; Lebrun, Luc. |
| The quality of oyster spermatozoa has become an issue for modern aquaculture. However, reliable protocols used to assess gamete quality are lacking. The aim of this work is to define a standardised experimental fertilisation protocol for Pacific oyster oocytes. Six experiments have been carried out in this study. The optimal conditions for Pacific oyster experimental fertilisation have been defined: (1) oocytes can be conserved in seawater for at least 4 h before fertilisation. (2) oocyte concentration: 100-1000 ml(-1) seawater, (3) fertilisation volume: 10-100 nil, (4) spermatozoa: oocyte ratio: 400 to maintain discriminating conditions, (5) gamete contact time: longer than 10 mill and (6) fertilisation and incubation temperatures: both 19 degrees C. The... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Pacific oyster; Experimental; Fertilisation. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6231.pdf |
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