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| Bellostas, Natalia; Casanova, Esther; Garcia-Mina, Jose Maria; Sorensen, Jens Christian; Sorensen, Hilmer. |
| Glucosinolates are allelochemicals present in all plants of the order Capparales that are hydrolysed by endogenous enzymes (myrosinases) forming a variety of compounds with biological activity. ‘Biofumigation’ is the term used to describe the effect of these compounds on soil-borne pathogens and it has normally been attributed to isothiocyanates. At acidic pH and in the presence of redox co-factors such as glutathione, glucosinolate hydrolysis yields also nitriles, which are more hydrophilic and stable than isothiocyanates. Three nitriles (allyl-, benzyl- and phenethyl cyanide) were tested against soil borne fungi of economic importance: Aphanomyces euteiches var. pisi, Gaeumannomyces graminis var. tritici and Verticillium dahliae. The nitriles were... |
| Tipo: Conference paper, poster, etc. |
Palavras-chave: Crop health; Quality; Protection. |
| Ano: 2006 |
URL: http://orgprints.org/9261/1/9261.pdf |
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| Bellostas, Natalia; Kudsk, Per; Sorensen, Jens Christian; Sorensen, Hilmer. |
| Glucosinolates are allelochemicals present in all Brassica plants. Upon hydrolysis by endogenous enzymes they produce a series of biologically active compounds, such as isothiocyanates and their deriva-tives among others. These compounds have marked fungicidal, nematocidal and herbicidal effects and therefore their use as biodegradable natural products for crop protection has attracted much attention in the last years. A number of these compounds, either individually or in combination, were tested against Sinapis alba and Lollium perenne in Petri dishes bio-assays. C50 values as low as 0.7 and 0.2 mM were obtained. This may open the possibility for using glucosinolate hydrolysis products as herbicides. |
| Tipo: Conference paper, poster, etc. |
Palavras-chave: Weed management. |
| Ano: 2006 |
URL: http://orgprints.org/9266/1/9266.pdf |
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| Bellostas, Natalia; Kachlicki, Piotr; Sorensen, Jens Christian; Sorensen, Hilmer. |
| Brassicaceous food crops contain in their tissues different quantities of the glucoside allelochemicals known as glucosinolates (Bellostas et al., 2004; Sørensen, 1990). These compounds are alkyl-N-hydroximine sulphate esters with a β-D-thioglucopyranoside group attached to the hydroximine carbon in Z-configuration relative to the sulphate group (Ettlinger and Kjær, 1968; Kjær, 1960). Glucosinolates are biosynthetically derived from amino acids (Hill et al., 2003) and they occur in all plants of the order Capparales and in some other plants (Bjerg and Sørensen, 1987; Kjær, 1960; Rodman, 1978). These compounds co-occur with myrosinase isoenzymes (Thioglucosidase; EC 3.2.1.147), which catalyze the hydrolysis of the β-D-thioglucopyranoside bond releasing an... |
| Tipo: Conference paper, poster, etc. |
Palavras-chave: Food security; Food quality and human health. |
| Ano: 2005 |
URL: http://orgprints.org/9267/1/9267.pdf |
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| Bellostas, Natalia; Sorensen, Jens Christian; Sorensen, Hilmer. |
| Biofumigation is based on the use of glucosinolate-containing plants for the control of soil-borne pest and diseases. Upon tissue damage, glucosinolates are hydrolyzed by endogenous enzymes (myrosinase) and a range of biologically active compounds are formed. Isothiocyanates (ITCs) are the quantitatively dominating products formed at neutral pH. Most of these compounds are volatile and only sparingly soluble in aqueous systems, and depending on the R-group structure and the presence of nucleophiles, further transformation of ITCs occurs. At lower pH and in the presence of certain molecules able to deliver two redox equivalents, the proportion of nitriles increases at the expense of ITC. The effect of ascorbic acid and glutathione on the production of... |
| Tipo: Conference paper, poster, etc. |
Palavras-chave: Crop health; Quality; Protection Food security; Food quality and human health. |
| Ano: 2006 |
URL: http://orgprints.org/9263/1/9263.pdf |
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| Bellostas, Natalia; Sorensen, Jens Christian; Sorensen, Hilmer. |
| A micellar electrokinetic capillary chromatography (MECC) method has been developed for monitoring the myrosinase catalysed hydrolysis of 2-hydroxy substituted glucosinolates and the simultaneous formation of the corresponding degradation products (oxazolidine-2-thiones (OZTs) and nitriles). Glucosibarin ((2R)-2-hydroxy-2-phenylethylglucosinolate) was chosen as the model glucosinolate owing to the difficulties in determining hydrolysis rates of this type of substrates in traditional UV-assays. The method was afterwards validated with glucobarbarin ((2S)-2-hydroxy-2-phenylethylglucosinolate) and progoitrin ((2R)-2-hydroxybut-3-enylglucosinolate). Aromatic glucosinolates without a 2-hydroxy group in their side chains, such as glucotropaeolin... |
| Tipo: Journal paper |
Palavras-chave: Crop health; Quality; Protection Food security; Food quality and human health. |
| Ano: 2006 |
URL: http://orgprints.org/9260/1/9260.pdf |
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