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Growth, sporulation and production of bioactive compounds by Bacillus subtilis R14 BABT
Carvalho,André Lacerda Ulysses de; Oliveira,Fábio Henrique Portella Corrêa de; Mariano,Rosa de Lima Ramos; Gouveia,Ester Ribeiro; Souto-Maior,Ana Maria.
The physiology of B. subtilis R14 was investigated in minimal medium under excess-oxygen and oxygen-limited conditions. Growth and efficient sporulation could be achieved in excess-oxygen culture on medium with readily metabolizable carbon and nitrogen sources, which allowed high growth rate and high biomass yield. A short transition phase between the exponential growth and sporulation could be attained by formulating a medium with a well-balanced C/N ratio. Under oxygen-limitation, but in the presence of essential nutrients (i.e. excess-nutrient cultivation), B. subtilis R14 produced bioactive compounds, which showed activity against several phytopathogenic bacteria. Under anaerobic condition, the organism did not grow neither through fermentation nor...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus subtilis; Batch cultivation; Oxygen availability; Antimicrobial activity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300020
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Anthracycline production by Streptomyces capoamus in batch fermentation BABT
Martins,Cristiane Soares; Souto-Maior,Ana Maria.
Anthracyclines have been intensely studied worldwide due to their importance as anticancer agents. In this work investigation was made on the production of ciclamycin, an antitumour complex of the anthracycline class, produced by Streptomyces capoamus. The fermentation medium consisted of glucose, as the main carbon source, and soymilk, as the sole nitrogen source. Experiments were performed in a 5-liter batch bioreactor under nitrogen and carbon-limiting conditions. Increasing antibiotic activity was observed both intra and extracellularly during growth under the two conditions used. Progressive loss of activity occurred in both cases after glucose was depleted from the medium. The results obtained showed that harvest of ciclamycin in a batch process...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthracycline; Ciclamycin; Streptomyces capoamus; Batch fermentation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300021
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Antagonism of Bacillus spp. against Xanthomonas campestris pv. campestris BABT
Monteiro,Leila; Mariano,Rosa de Lima Ramos; Souto-Maior,Ana Maria.
The antagonism of eight Bacillus isolates was investigated against nine strains of Xanthomonas campestris pv. campestris (causal agent of crucifers black rot) to assess the role of lipopeptides in this process. Antimicrobial and hemolytic (surfactant) activity tests were performed in vitro using agar diffusion methods. Antibiosis and hemolysis were positive for four Bacillus isolates against all X. campestris pv. campestris strains. The correlation observed between antimicrobial and hemolytic activities indicated that lipopeptides were involved in the antibiosis mechanism of the studied antagonists. Fermentation studies were carried out with the isolates that showed highest antimicrobial and hemolytic activities, to follow up growth and production of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Biological control; Xanthomonas campestris pv. campestris; Crucifers black rot.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100004
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