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Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques International Journal of Morphology
Catroxo,M. H. B; Martins,A. M. C. P. F; Petrella,S; Milanelo,L; Aschar,M; Souza,F; Nastari,B.D.B; Souza,R.B.
Diseases caused by avian paramyxovirus (APMV) occur in commercial, captive and wild birds worldwide, demonstrating the significant economic and ecological importance of these agents. Paramyxoviruses belong to the Paramyxoviridae family, Paramyxovirinae subfamily and Avulavirus genus. During the period 2000 to 2011, stool and small intestine samples of 1647 birds species were sent to the Laboratory of Electron Microscopy, Biological Institute of São Paulo, Brazil, for diagnosis of viral agents. The samples were processed by negative staining (rapid preparation) and resin embedding techniques. Under the transmission electron microscope by negative staining technique, in 294 (17.8%) samples of 1647 were visualized paramyxovirus particles pleomorphic, roughly...
Tipo: Journal article Palavras-chave: Paramyxoviruses; Avian; Transmission Electron Microscopy.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200062
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Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil International Journal of Morphology
Catroxo,M. H. B; Martins,A. M. C. R. P. F; Petrella,S; Souza,F; Nastari,B.D.B.
Papillomaviruses, belonging to the Papillomaviridae family, are small oncogenic viruses, causing papillomas and fibropapillomas in the mucosal and cutaneous epithelia of several animals. In bovine species, thirteen types (BPV 1-13) were characterized to date. In this study, the occurrence of papillomatosis in four outbreaks in cattle herds, coming from Brazilian states were registered. The papillomatous lesions were found located in the teats, udders, head and neck. Under the transmission electron microscope, by the negative staining technique, it was possible to visualized rounded-format papillomavirus, with icosahedral symmetry, characterized as "full" and "empty" particles, measuring on average 60 nm in diameter, in all the 40 samples observed of skin...
Tipo: Journal article Palavras-chave: Papillomavirus; Cattle; Transmission Electron Microscopy.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200068
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