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| Sousa,Diego Rayan Teixeira de; Santos,Carla Silvana da Silva; Wanke,Bodo; Silva Júnior,Roberto Moreira da; Santos,Marcelo Cordeiro dos; Cruz,Kátia Santana; Monte,Rossicléia Lins; Nocker,Andreas; Souza,João Vicente Braga de. |
| Background The incidence of invasive mycoses is increasing worldwide. PCR-RFLP was applied to the identification of 10 reference strains and 90 cultures of agents of invasive mycoses. In addition, the new approach was applied to detect fungal agents in 120 biological samples (blood, cerebrospinal fluid and bone marrow). PCR-RFLP results were compared with the ones obtained with conventional methods (culture, microscopy, and biochemical testing). Results The assays carried out with the reference strains (Candida albicans, Candida parapsilosis, Candida tropicalis, Candida krusei, Candida guilliermondii, Cryptococcus neoformans, Cryptococcus gattii and Histoplasma capsulatum), demonstrated that the RFLP profiles were correctly predicted by the in silico... |
| Tipo: Journal article |
Palavras-chave: Pathogenic fungus; Polymorphism; Simultaneous analysis. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300014 |
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| Lima,Alita Moura de; Salem,Júlia Ignez; Souza,João Vicente Braga de; Cortez,Ana Claudia Alves; Carvalho,Clarice Maia; Chaves,Francisco Célio Maia; Veiga Junior,Valdir Florêncio da. |
| Substances that inhibit the growth of Mycobacterium tuberculosis could potentially be used as antibiotics. These substances could also be added to test culture media to improve the speed of tuberculosis diagnosis. The aim of this work was to investigate the influence of culture filtrates of endophytic fungi isolated from P. aduncum L. on the growth of M. tuberculosis. To achieve this objective, the following methodology was used: a) endophytic fungi were isolated from the leaves and stems of P. aduncum L.; b) the isolated fungi were submitted to submerged bioprocessing; c) culture filtrates from the bioprocess were assayed to evaluate their effect on the growth of M. tuberculosis. We isolated 315 fungal types, which represented 85 morphologies, from... |
| Tipo: Journal article |
Palavras-chave: Antagonism; Stimulation growth; Tuberculosis. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400008 |
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