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| Sousa,A.L.M.; Pinheiro,R.R.; Araújo,J.F.; Santos,V.W.S.; Azevedo,D.A.A.; Peixoto,R.M.; Souza,V.; Andrioli,A.; Damasceno,E.M.; Dantas,T.V.M.; Teixeira,M.F.S.. |
| ABSTRACT The aim of this study was to evaluate in vitro and in vivo the effect of sodium dodecyl sulfate (SDS) on the caprine lentivirus (CLV) in colostrum and milk. This was performed to develop a practical and efficient method of blocking the lactogenic transmission of the virus. In the in vitro experiment, colostrum and milk were treated with 0.25%; 0.50% and 1% SDS. Then, somatic cells of colostrum and milk were submitted to co-culture with caprine synovial membrane cells (CSM). In the in vivo test, goats were fed with colostrum and milk provided from CLV-positive goats treated with SDS in the same concentrations used in the in vitro experiment. Animals were tested by nested polymerase chain reaction (nPCR) and Western blot (WB) assays. In the in vitro... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Monocyte-phagocytic system; Viral inactivation; Surfactant; Small ruminant lentiviruses. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501459 |
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| Souza,V.; Ordine,Alberto Pires; Fraga,I. C. S.; Getrouw,Monique Audrey; Borges,P. P.; Damasceno,J. C.; Couto,P. R. G.. |
| pH is an important parameter to control the production of food, medicine, petrochemical products and others. Thus, standard reference materials used to calibrate pH meters are necessary to guarantee reliability of the measurements. The Chemical Metrology Division (Dquim) of the National Metrology Institute of Brazil (Inmetro) has as one of its missions the certification of standard reference materials. In the case of pH, it will be done by the pH primary measurement system of Inmetro, in operation at Dquim since 2003. The buffer solution of nominal pH value equal to 6.865 will be the first to be certified. The study of some measurement parameters has already been accomplished, and will lead to the improvement of the primary pH measurement. In this paper,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chemical metrology; Primary pH; Reference material. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200013 |
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