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| Pinheiro,Cassia Renata; Amorim,Julie Anne Espíndola; Diniz,Leandro Eugenio Cardamone; Silva,Adriano Márcio Freire da; Talamini,Viviane; Souza Júnior,Manoel Teixeira. |
| O objetivo deste trabalho foi identificar isolados brasileiros de Ralstonia solanacearum quanto a filotipos e sequevares, determinar sua diversidade genética, realizar a associação da estrutura genética do patógeno com sua classificação e origem geográfica e identificar um marcador molecular para a diagnose do moko-da-bananeira. Um grupo de 33 isolados de R. solanacearum, da coleção da Embrapa Tabuleiros Costeiros, coletado de diversos hospedeiros, foi caracterizado por meio de PCR em sequência palindrômica extragênica repetitiva (rep-PCR) e RAPD. Deste grupo, 19 perteciam à raça 2 do patógeno e 14 à raça 1, e 15 isolados eram associados à cultura da bananeira. Os filotipos e sequevares foram caracterizados e determinados por PCR Multiplex. Verificou-se... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musa; Marcador molecular; Moko-da-bananeira; Murcha bacteriana; Rep-PCR; Variabilidade genética. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000600004 |
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| Souza Júnior,Manoel Teixeira; Gonsalves,Dennis. |
| The Papaya ringspot virus (PRSV) coat protein transgene present in 'Rainbow' and 'SunUp' papayas disclose high sequence similarity (>89%) to the cp gene from PRSV BR and TH. Despite this, both isolates are able to break down the resistance in 'Rainbow', while only the latter is able to do so in 'SunUp'. The objective of this work was to evaluate the degree of sequence similarity between the cp gene in the challenge isolate and the cp transgene in transgenic papayas resistant to PRSV. The production of a hybrid virus containing the genome backbone of PRSV HA up to the Apa I site in the NIb gene, and downstream from there, the sequence of PRSV TH was undertaken. This hybrid virus, PRSV HA/TH, was obtained and used to challenge 'Rainbow', 'SunUp', and an... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya; Potyvirus; Resistance; Breeding; Virus. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000500009 |
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| Capdeville,Guy de; Souza Júnior,Manoel Teixeira; Santos,Jansen Rodrigo Pereira; Miranda,Simoni Paula; Caetano,Alexandre Rodrigues; Falcão,Rosana; Gomes,Ana Cristina Menezes Mendes. |
| The objective of this work was to investigate possible modes of action of the yeast Cryptococcus magnus in controlling anthracnose (Colletotrichum gloeosporioides) on post harvested papaya fruits. Scanning electron microscopy was used to analyze the effect of the yeast on inoculations done after harvest. Results showed that C. magnus is able to colonize wound surfaces much faster than the pathogen, outcompeting the later for space and probably for nutrients. In addition, C. magnus produces a flocculent matrix, which affects hyphae integrity. The competition for space and the production of substances that affect hyphae integrity are among the most important modes of action of this yeast. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya; Anthracnose; Antagonist yeast; Biological control; Competition. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007001100004 |
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